Academic Journal
Assessment of DNA Damage after Photodynamic Therapy Using a Metallophthalocyanine Photosensitizer
| العنوان: | Assessment of DNA Damage after Photodynamic Therapy Using a Metallophthalocyanine Photosensitizer |
|---|---|
| المؤلفون: | A. El-Hussein, M. Harith, H. Abrahamse |
| المصدر: | International Journal of Photoenergy, Vol 2012 (2012) |
| بيانات النشر: | Hindawi Limited, 2012. |
| سنة النشر: | 2012 |
| المجموعة: | LCC:Renewable energy sources |
| مصطلحات موضوعية: | Renewable energy sources, TJ807-830 |
| الوصف: | Photodynamic therapy (PDT) is a chemotherapeutic approach that utilizes a bifunctional reagent, a photosensitizer (PS) that localizes to the target tissue relative to the surrounding tissue and is toxic when exposed to laser light. PDT rapidly induces cell death, inflammatory and immune reactions, and damage of the microvasculature. DNA damage results from a variety of factors including UV-light, X-rays, ionizing radiation, toxins, chemicals, or reactive oxygen species. The aim of this study was to determine the effect of PDT as well as the influence of presensitization leading to the adaptive response (AR) on the integrity of DNA. Lung (A549), breast (MCF-7), and esophageal (SNO) cancer cells and Zn sulfophthalocyanine as PS with irradiation conditions of 10 J/cm2 at 636 nm were used. Subcellular localization of PS, cell morphology, and viability after PDT and DNA damage were determined. A significant decrease in viability and marked DNA damage was observed in all 3 cancer cell types in response to PDT while the adaptive response was demonstrated to significantly decrease the effectiveness of the PDT. |
| نوع الوثيقة: | article |
| وصف الملف: | electronic resource |
| اللغة: | English |
| تدمد: | 1110-662X 1687-529X |
| Relation: | https://doaj.org/toc/1110-662X; https://doaj.org/toc/1687-529X |
| DOI: | 10.1155/2012/281068 |
| URL الوصول: | https://doaj.org/article/3ad20733f19a424a89c3f2965ed47cf5 |
| رقم الانضمام: | edsdoj.3ad20733f19a424a89c3f2965ed47cf5 |
| قاعدة البيانات: | Directory of Open Access Journals |
Full Text Finder | تدمد: | 1110662X 1687529X |
|---|---|
| DOI: | 10.1155/2012/281068 |