Academic Journal
The Effect of Doxycycline on PMA-Induced MUC5B Expression via MMP-9 and p38 in NCI-H292 Cells
| العنوان: | The Effect of Doxycycline on PMA-Induced MUC5B Expression via MMP-9 and p38 in NCI-H292 Cells |
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| المؤلفون: | Chang Hoon Bae, Seung Min Chen, Heung-Man Lee, Si-Youn Song, Yong-Dae Kim |
| المصدر: | Clinical and Experimental Otorhinolaryngology, Vol 4, Iss 4, Pp 177-183 (2011) |
| بيانات النشر: | Korean Society of Otorhinolaryngology-Head and Neck Surgery, 2011. |
| سنة النشر: | 2011 |
| المجموعة: | LCC:Medicine LCC:Otorhinolaryngology |
| مصطلحات موضوعية: | Doxycycline, Inflammation, Phorbol myristate acetate, Matrix metalloproteinase-9, p38, Mucins, MUC5B, Epithelial cells, NCI-H292 cell, Medicine, Otorhinolaryngology, RF1-547 |
| الوصف: | ObjectivesDoxycycline is commonly used in medicine for its bacteriostatic antimicrobial properties. Recent studies have reported that doxycycline also has anti-inflammatory effects. Matrix metalloproteinase (MMP)-9 has been found to be involved in the physiological and pathological process of inflammatory airway disease. Phorbol 12-myristate 13-acetate (PMA), a protein kinase C activator, is known to stimulate the expression of MMP and mucin genes in the airway and intestinal epithelial cells. Therefore, the effects and signal pathways of doxycycline on PMA-induced MUC5B expression dependent MMP-9 in human airway epithelial cells were investigated.MethodsIn human NCI-H292 airway epithelial cells, MUC5B and MMP-9 mRNA expression, MUC5B protein expression, and MMP-9 protein activity after the treatment with PMA, MMP-9 or doxycycline were determined by reverse transcriptase-polymerase chain reaction, enzyme immunoassay, gelatin zymography, and Western blot analysis.ResultsPMA increased MMP-9 and MUC5B expression. MMP-9 increased MUC5B expression. Doxycycline inhibited PMA-induced MUC5B expression, and PMA-induced MMP-9 mRNA expression and protein activity. Doxycycline inhibited phosphorylation of p38 induced by PMA and MMP-9.ConclusionThe results of this study suggest that doxycycline inhibited PMA-induced MUC5B mRNA expression and protein production through the MMP-9 and p38 pathways in human NCI-H292 airway epithelial cells. |
| نوع الوثيقة: | article |
| وصف الملف: | electronic resource |
| اللغة: | English |
| تدمد: | 1976-8710 2005-0720 |
| Relation: | http://www.e-ceo.org/upload/pdf/ceo-4-177.pdf; https://doaj.org/toc/1976-8710; https://doaj.org/toc/2005-0720 |
| DOI: | 10.3342/ceo.2011.4.4.177 |
| URL الوصول: | https://doaj.org/article/38df5911cdd7449ba4095fb2bc3f30bf |
| رقم الانضمام: | edsdoj.38df5911cdd7449ba4095fb2bc3f30bf |
| قاعدة البيانات: | Directory of Open Access Journals |
Full Text Finder | تدمد: | 19768710 20050720 |
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| DOI: | 10.3342/ceo.2011.4.4.177 |