التفاصيل البيبلوغرافية
| العنوان: |
Excess Glucose Impedes the Proliferation of Skeletal Muscle Satellite Cells Under Adherent Culture Conditions |
| المؤلفون: |
Yasuro Furuichi, Yuki Kawabata, Miho Aoki, Yoshitaka Mita, Nobuharu L. Fujii, Yasuko Manabe |
| المصدر: |
Frontiers in Cell and Developmental Biology, Vol 9 (2021) |
| بيانات النشر: |
Frontiers Media S.A., 2021. |
| سنة النشر: |
2021 |
| المجموعة: |
LCC:Biology (General) |
| مصطلحات موضوعية: |
satellite cell, proliferation, self-renewal, glucose, primary culture, Biology (General), QH301-705.5 |
| الوصف: |
Glucose is a major energy source consumed by proliferating mammalian cells. Therefore, in general, proliferating cells have the preference of high glucose contents in extracellular environment. Here, we showed that high glucose concentrations impede the proliferation of satellite cells, which are muscle-specific stem cells, under adherent culture conditions. We found that the proliferation activity of satellite cells was higher in glucose-free DMEM growth medium (low-glucose medium with a glucose concentration of 2 mM) than in standard glucose DMEM (high-glucose medium with a glucose concentration of 19 mM). Satellite cells cultured in the high-glucose medium showed a decreased population of reserve cells, identified by staining for Pax7 expression, suggesting that glucose concentration affects cell fate determination. In conclusion, glucose is a factor that decides the cell fate of skeletal muscle-specific stem cells. Due to this unique feature of satellite cells, hyperglycemia may negatively affect the regenerative capability of skeletal muscle myofibers and thus facilitate sarcopenia. |
| نوع الوثيقة: |
article |
| وصف الملف: |
electronic resource |
| اللغة: |
English |
| تدمد: |
2296-634X |
| Relation: |
https://www.frontiersin.org/articles/10.3389/fcell.2021.640399/full; https://doaj.org/toc/2296-634X |
| DOI: |
10.3389/fcell.2021.640399 |
| URL الوصول: |
https://doaj.org/article/c38c5063472e45109903203f362f8527 |
| رقم الانضمام: |
edsdoj.38c5063472e45109903203f362f8527 |
| قاعدة البيانات: |
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