التفاصيل البيبلوغرافية
| العنوان: |
Upregulation of Epithelial-To-Mesenchymal Transition Markers and P2X7 Receptors Is Associated to Increased Invasiveness Caused by P2X7 Receptor Stimulation in Human Glioblastoma Stem Cells |
| المؤلفون: |
Sihana Ziberi, Mariachiara Zuccarini, Marzia Carluccio, Patricia Giuliani, Lucia Ricci-Vitiani, Roberto Pallini, Francesco Caciagli, Patrizia Di Iorio, Renata Ciccarelli |
| المصدر: |
Cells, Vol 9, Iss 1, p 85 (2019) |
| بيانات النشر: |
MDPI AG, 2019. |
| سنة النشر: |
2019 |
| المجموعة: |
LCC:Cytology |
| مصطلحات موضوعية: |
glioblastoma stem cells (gscs), epithelial-to-mesenchymal transition (emt) markers, gsc invasiveness, transforming growth factor beta, bzatp, p2x7 receptor splice variants a and b, Cytology, QH573-671 |
| الوصف: |
Glioblastoma (GBM) stem cells (GSCs), which contribute to GBM unfavorable prognosis, show high expression levels of ATP/P2X7 receptors (P2X7R). Here, we reported that cells exposure to 2’(3’)-O-(4-benzoylbenzoyl)-ATP (BzATP), a P2X7R agonist, up-regulated the expression of markers associated to epithelial-to-mesenchymal transition (EMT), a process likely contributing to GSC malignancy, and increased GSC migration/invasiveness like the known EMT inducer, Transforming Growth Factor β1 (TGFβ1). These effects were coupled to phosphorylation of SMAD2, a downstream effector in the TGFβ pathway, suggesting its involvement in P2X7R-mediated activity in GSCs. All BzATP effects, including a decrease in the caspase 3/7 activity in GSC medium, were mostly counteracted by the P2X7R antagonist A438079. Finally, BzATP increased the subunit expression of two main human P2X7R splice variants, the full-length P2X7A and the truncated P2X7B, lacking the carboxylic tail, which have different functional properties depending on their arrangement. Since up-regulation of A/B subunits might favor their assembly into a heterotrimeric P2X7R with great sensitivity towards agonists and cell energy support, this is in line with increased EMT markers expression, cell migration/invasion and GSC survival observed following P2X7R stimulation. As in GBM microenvironment extracellular ATP levels may activate P2X7R, our data suggest a P2X7R role in GBM recurrence/invasiveness. |
| نوع الوثيقة: |
article |
| وصف الملف: |
electronic resource |
| اللغة: |
English |
| تدمد: |
2073-4409 |
| Relation: |
https://www.mdpi.com/2073-4409/9/1/85; https://doaj.org/toc/2073-4409 |
| DOI: |
10.3390/cells9010085 |
| URL الوصول: |
https://doaj.org/article/375d69bbfba841d1ae6180d025efc026 |
| رقم الانضمام: |
edsdoj.375d69bbfba841d1ae6180d025efc026 |
| قاعدة البيانات: |
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