Academic Journal
Triple immunofluorescence labeling of atherosclerotic plaque components in apoE/LDLR -/- mice.
| العنوان: | Triple immunofluorescence labeling of atherosclerotic plaque components in apoE/LDLR -/- mice. |
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| المؤلفون: | Stefan Chłopicki, Andrzej Radziszewski, Grzegorz J Lis, Lukasz Mateuszuk, Jacek Jawień, Mariusz Gajda, Jan A Litwin |
| المصدر: | Folia Histochemica et Cytobiologica, Vol 46, Iss 2, Pp 143-146 (2008) |
| بيانات النشر: | Via Medica, 2008. |
| سنة النشر: | 2008 |
| المجموعة: | LCC:Cytology |
| مصطلحات موضوعية: | Cytology, QH573-671 |
| الوصف: | This paper presents a simple and reliable method of triple immunofluorescence staining that allows simultaneous detection of various cell types present in atherosclerotic plaque of apolipoprotein E and LDL receptor-double knockout (apoE/LDLR -/-) mice. We used combined direct and indirect procedures applying commercially available primary antibodies raised in different species to detect smooth muscle cells (Cy3-conjugated mouse anti-smooth muscle actin, SMA), macrophages (rat anti-CD68) and T lymphocytes (rabbit anti-CD3). Fixation of the material in acetone and modified incubation protocol employing nonfat dry milk in preincubation and incubation media significantly increased the intensity of labeling and effectively quenched the background. Our method offers an efficient way to detect qualitative as well as quantitative changes of macrophages, T lymphocytes and smooth muscle cells in atherosclerotic plaque of apoE/LDLR -/- mice during atherosclerosis development or in response to pharmacological treatment. |
| نوع الوثيقة: | article |
| وصف الملف: | electronic resource |
| اللغة: | English |
| تدمد: | 0239-8508 1897-5631 |
| Relation: | http://czasopisma.viamedica.pl/fhc/article/view/4449; https://doaj.org/toc/0239-8508; https://doaj.org/toc/1897-5631 |
| DOI: | 10.5603/4449 |
| URL الوصول: | https://doaj.org/article/c36664cd436a4e659b63a4c77882f373 |
| رقم الانضمام: | edsdoj.36664cd436a4e659b63a4c77882f373 |
| قاعدة البيانات: | Directory of Open Access Journals |
| تدمد: | 02398508 18975631 |
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| DOI: | 10.5603/4449 |