التفاصيل البيبلوغرافية
| العنوان: |
Pressure-Retaining Sampler and High-Pressure Systems to Study Deep-Sea Microbes Under in situ Conditions |
| المؤلفون: |
Marc Garel, Patricia Bonin, Séverine Martini, Sophie Guasco, Marie Roumagnac, Nagib Bhairy, Fabrice Armougom, Christian Tamburini |
| المصدر: |
Frontiers in Microbiology, Vol 10 (2019) |
| بيانات النشر: |
Frontiers Media S.A., 2019. |
| سنة النشر: |
2019 |
| المجموعة: |
LCC:Microbiology |
| مصطلحات موضوعية: |
pressure-retaining sampler, deep sea, in situ sampling, prokaryotic activities, prokaryotic diversity, Microbiology, QR1-502 |
| الوصف: |
The pelagic realm of the dark ocean is characterized by high hydrostatic pressure, low temperature, high-inorganic nutrients, and low organic carbon concentrations. Measurements of metabolic activities of bathypelagic bacteria are often underestimated due to the technological limitations in recovering samples and maintaining them under in situ environmental conditions. Moreover, most of the pressure-retaining samplers, developed by a number of different labs, able to maintain seawater samples at in situ pressure during recovery have remained at the prototype stage, and therefore not available to the scientific community. In this paper, we will describe a ready-to-use pressure-retaining sampler, which can be adapted to use on a CTD-carousel sampler. As well as being able to recover samples under in situ high pressure (up to 60 MPa) we propose a sample processing in equi-pressure mode. Using a piloted pressure generator, we present how to perform sub-sampling and transfer of samples in equi-pressure mode to obtain replicates and perform hyperbaric experiments safely and efficiently (with |
| نوع الوثيقة: |
article |
| وصف الملف: |
electronic resource |
| اللغة: |
English |
| تدمد: |
1664-302X |
| Relation: |
https://www.frontiersin.org/article/10.3389/fmicb.2019.00453/full; https://doaj.org/toc/1664-302X |
| DOI: |
10.3389/fmicb.2019.00453 |
| URL الوصول: |
https://doaj.org/article/3625e2f12efa49e5aba08b15c6a09709 |
| رقم الانضمام: |
edsdoj.3625e2f12efa49e5aba08b15c6a09709 |
| قاعدة البيانات: |
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