التفاصيل البيبلوغرافية
| العنوان: |
Parallel detection of multiple zoonotic parasites using a real-time fluorogenic loop-mediated isothermal amplification-based quadruple-sample microfluidic chip |
| المؤلفون: |
Yu-Xin Chen, Yi-Rong Lou, Li-Jun Duan, Qian-Jin Zhou, Zhong-Jie Xu, Fang-Jie Chen, Hong-Xian Chen, Gui-Zong Xu, Ai-Fang Du, Jiong Chen |
| المصدر: |
Frontiers in Microbiology, Vol 14 (2023) |
| بيانات النشر: |
Frontiers Media S.A., 2023. |
| سنة النشر: |
2023 |
| المجموعة: |
LCC:Microbiology |
| مصطلحات موضوعية: |
zoonotic parasite, microfluidic chip, loop-mediated isothermal amplification, low reagent consumption, multiple detection, Microbiology, QR1-502 |
| الوصف: |
Zoonotic parasites pose significant health risks globally. In the present study, we combined a microfluidic chip with loop-mediated isothermal amplification (on-chip LAMP) to detect five zoonotic parasites: Toxoplasma gondii, Cryptosporidium parvum, Cryptosporidium hominis, Clonorchis sinensis, and Taenia solium. This method enabled the simultaneous parallel analysis of five genetic markers from a maximum of four samples per chip. The on-chip LAMP assay was conducted in a highly automated format via the addition (by pipetting) of each sample in a single operation. The reaction was performed in volumes as low as 5 μL at a temperature of 65°C for 60 min, achieving limits of detection ranging from 10−2 to 10−3 pg./μL of recombinant plasmid DNA. All the time-to-positive values were less than 40 min, and almost all the coefficients of variation were less than 10%, even when using limit of detection concentrations for multiple pathogens, indicating robust reproducibility among replicates. The clinical sensitivity and specificity for detecting 135 field samples were 98.08 and 97.59%, respectively, compared with traditional biological methods, indicating good applicability in the detection of field samples. This on-chip LAMP assay allows for low reagent consumption, ease of operation, and multiple analyses of samples and genetic targets, and is applicable for on-site detection and the routine monitoring of multiple zoonotic parasites. |
| نوع الوثيقة: |
article |
| وصف الملف: |
electronic resource |
| اللغة: |
English |
| تدمد: |
1664-302X |
| Relation: |
https://www.frontiersin.org/articles/10.3389/fmicb.2023.1238376/full; https://doaj.org/toc/1664-302X |
| DOI: |
10.3389/fmicb.2023.1238376 |
| URL الوصول: |
https://doaj.org/article/3600853452244ea68f6b348250f951d6 |
| رقم الانضمام: |
edsdoj.3600853452244ea68f6b348250f951d6 |
| قاعدة البيانات: |
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