Academic Journal
Bioprospecting of novel thermostable β-glucosidase from Bacillus subtilis RA10 and its application in biomass hydrolysis
| العنوان: | Bioprospecting of novel thermostable β-glucosidase from Bacillus subtilis RA10 and its application in biomass hydrolysis |
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| المؤلفون: | Rameshwar Tiwari, Puneet Kumar Singh, Surender Singh, Pawan K. S. Nain, Lata Nain, Pratyoosh Shukla |
| المصدر: | Biotechnology for Biofuels, Vol 10, Iss 1, Pp 1-18 (2017) |
| بيانات النشر: | BMC, 2017. |
| سنة النشر: | 2017 |
| المجموعة: | LCC:Fuel LCC:Biotechnology |
| مصطلحات موضوعية: | Thermotolerant, Thermostable β-glucosidase, Saccharification, Cloning and expression, Molecular modelling, Fuel, TP315-360, Biotechnology, TP248.13-248.65 |
| الوصف: | Abstract Background Saccharification is the most crucial and cost-intensive process in second generation biofuel production. The deficiency of β-glucosidase in commercial enzyme leads to incomplete biomass hydrolysis. The decomposition of biomass at high temperature environments leads us to isolate thermotolerant microbes with β-glucosidase production potential. Results A total of 11 isolates were obtained from compost and cow dung samples that were able to grow at 50 °C. On the basis of qualitative and quantitative estimation of β-glucosidase enzyme production, Bacillus subtilis RA10 was selected for further studies. The medium components and growth conditions were optimized and β-glucosidase enzyme production was enhanced up to 19.8-fold. The β-glucosidase from B. subtilis RA10 retained 78% of activity at 80 °C temperature and 68.32% of enzyme activity was stable even at 50 °C after 48 h of incubation. The supplementation of β-glucosidase from B. subtilis RA10 into commercial cellulase enzyme resulted in 1.34-fold higher glucose release. Furthermore, β-glucosidase was also functionally elucidated by cloning and overexpression of full length GH1 family β-glucosidase gene from B. subtilis RA10. The purified protein was characterized as thermostable β-glucosidase enzyme. Conclusions The thermostable β-glucosidase enzyme from B. subtilis RA10 would facilitate efficient saccharification of cellulosic biomass into fermentable sugar. Consequently, after saccharification, thermostable β-glucosidase enzyme would be recovered and reused to reduce the cost of overall bioethanol production process. |
| نوع الوثيقة: | article |
| وصف الملف: | electronic resource |
| اللغة: | English |
| تدمد: | 1754-6834 84076933 |
| Relation: | http://link.springer.com/article/10.1186/s13068-017-0932-8; https://doaj.org/toc/1754-6834 |
| DOI: | 10.1186/s13068-017-0932-8 |
| URL الوصول: | https://doaj.org/article/33d13d967bd84076933557486646e8dd |
| رقم الانضمام: | edsdoj.33d13d967bd84076933557486646e8dd |
| قاعدة البيانات: | Directory of Open Access Journals |
Full Text Finder | تدمد: | 17546834 84076933 |
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| DOI: | 10.1186/s13068-017-0932-8 |