Academic Journal
Principle and Application of Trans-Cleavage CRISPR/Cas Technology for the Detection of Foodborne Pathogenic Microorganisms
| العنوان: | Principle and Application of Trans-Cleavage CRISPR/Cas Technology for the Detection of Foodborne Pathogenic Microorganisms |
|---|---|
| المؤلفون: | DAI Yongjin, SUN Jing, ZHANG Moran, LIU Yujuan, CHEN Hongzhou, LU Yingjian |
| المصدر: | Shipin Kexue, Vol 45, Iss 22, Pp 351-360 (2024) |
| بيانات النشر: | China Food Publishing Company, 2024. |
| سنة النشر: | 2024 |
| المجموعة: | LCC:Food processing and manufacture |
| مصطلحات موضوعية: | clustered regularly interspaced short palindromic repeats (crispr)/crispr-associated proteins systems, trans-cleavage, foodborne pathogen, nucleic acid detection, Food processing and manufacture, TP368-456 |
| الوصف: | Foodborne pathogenic microorganisms are a serious threat to food safety. Currently, traditional biochemical assays are facing a series of challenges in the detection of foodborne pathogens. In recent years, with the gradual development of CRISPR (clustered regularly interspaced short palindromic repeats) technology, the CRISPR/CRISPR-associated proteins (Cas) system with trans-cleavage activity has received much attention from researchers. In this article, the origin, classification and principles of CRISPR/Cas systems with trans-cleavage activity are summarized. This paper also reviews the versatile application of trans-cleavage CRISPR/Cas systems in the field of foodborne pathogens, and concludes with a discussion of the advantages, disadvantages and future directions of this technology. |
| نوع الوثيقة: | article |
| وصف الملف: | electronic resource |
| اللغة: | English Chinese |
| تدمد: | 1002-6630 |
| Relation: | https://www.spkx.net.cn/fileup/1002-6630/PDF/2024-45-22-039.pdf; https://doaj.org/toc/1002-6630 |
| DOI: | 10.7506/spkx1002-6630-20231205-029 |
| URL الوصول: | https://doaj.org/article/33c7364473c04db4aaaa18d772750680 |
| رقم الانضمام: | edsdoj.33c7364473c04db4aaaa18d772750680 |
| قاعدة البيانات: | Directory of Open Access Journals |
| تدمد: | 10026630 |
|---|---|
| DOI: | 10.7506/spkx1002-6630-20231205-029 |