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An upstream open reading frame regulates LST1 expression during monocyte differentiation.

التفاصيل البيبلوغرافية
العنوان: An upstream open reading frame regulates LST1 expression during monocyte differentiation.
المؤلفون: Christian Schiller, Carina Nowak, Kalliope N Diakopoulos, Ulrich H Weidle, Elisabeth H Weiss
المصدر: PLoS ONE, Vol 9, Iss 5, p e96245 (2014)
بيانات النشر: Public Library of Science (PLoS), 2014.
سنة النشر: 2014
المجموعة: LCC:Medicine
LCC:Science
مصطلحات موضوعية: Medicine, Science
الوصف: The regulation of gene expression depends on the interplay of multiple factors at the transcriptional and translational level. Upstream open reading frames (uORFs) play an important role as translational repressors of main ORFs and their presence or usage in transcripts can be regulated by different mechanisms. The main objective of the present study was to assess whether uORFs regulate the expression of the MHC class III gene LST1. We report that expression of LST1 is tightly regulated by alternative transcription initiation and the presence of an uORF in the 5'-UTR of transcripts. Specifically, using EGFP reporter constructs in human HeLa and HEK-293T cells and flow cytometry as well as western blot analysis we found the uORF to reduce the expression of the main ORF by roughly two-thirds. Furthermore, we were able to correlate a previously detected increase in LST1 protein expression during monocyte differentiation with an increase of transcription initiation at an alternative exon that does not contain an uORF.
نوع الوثيقة: article
وصف الملف: electronic resource
اللغة: English
تدمد: 1932-6203
Relation: http://europepmc.org/articles/PMC4015914?pdf=render; https://doaj.org/toc/1932-6203
DOI: 10.1371/journal.pone.0096245
URL الوصول: https://doaj.org/article/32f3549676ea4e4bacc07f3362de9940
رقم الانضمام: edsdoj.32f3549676ea4e4bacc07f3362de9940
قاعدة البيانات: Directory of Open Access Journals
الوصف
تدمد:19326203
DOI:10.1371/journal.pone.0096245