التفاصيل البيبلوغرافية
| العنوان: |
Crystallization and Characterization of an Inflammatory Lectin Purified from the Seeds of Dioclea wilsonii |
| المؤلفون: |
Ana Maria Sampaio Assreuy, Alana de Freitas Pires, Amanda Uliana de Carvalho, Raquel Guimarães Benevides, Rafael da Conceição Simões, Helton Colares da Silva, Maria Júlia Barbosa Bezerra, Antonia Samia Fernandes do Nascimento, Kyria Santiago do Nascimento, Celso Shiniti Nagano, Alexandre Holanda Sampaio, Plínio Delatorre, Bruno Anderson Matias da Rocha, Patricia Machado Bueno Fernandes, Benildo Sousa Cavada, Thaiz Batista Azevedo Rangel |
| المصدر: |
Molecules, Vol 16, Iss 6, Pp 5087-5103 (2011) |
| بيانات النشر: |
MDPI AG, 2011. |
| سنة النشر: |
2011 |
| المجموعة: |
LCC:Organic chemistry |
| مصطلحات موضوعية: |
crystallization, Dioclea wilsonii, inflammation, lectin, tandem mass spectrometry, Organic chemistry, QD241-441 |
| الوصف: |
DwL, a lectin extracted from the seeds of Dioclea wilsonii, is a metalloprotein with strong agglutinating activity against rabbit and ABO erythrocytes, inhibited by glucose and mannose. DwL was purified by affinity chromatography on a Sephadex G-50 column and ion exchange chromatography on a HiTrap SP XL column. SDS-PAGE revealed three electrophoretic bands corresponding to the α (25,634 ± 2 Da), β (12,873 ± 2 Da) and γ (12,779 ± 2 Da) chains. Protein sequencing was done by Tandem Mass Spectrometry. The primary sequence featured 237 amino acids and was highly homologous to other reported Diocleinae lectins. A complete X-ray dataset was collected at 2.0 Å for X-Man-complexed DWL crystals produced by the vapor diffusion method. The crystals were orthorhombic and belonged to the space group I222, with the unit-cell parameters a = 59.6, b = 67.9 and c = 109.0 Å. DWL differed in potency from other ConA-like lectins and was found to induce neutrophil migration in rats, making it particularly useful in structural/functional studies of this class of proteins. |
| نوع الوثيقة: |
article |
| وصف الملف: |
electronic resource |
| اللغة: |
English |
| تدمد: |
1420-3049 |
| Relation: |
http://www.mdpi.com/1420-3049/16/6/5087/; https://doaj.org/toc/1420-3049 |
| DOI: |
10.3390/molecules16065087 |
| URL الوصول: |
https://doaj.org/article/329e364e593b470d835d8c6ce807099e |
| رقم الانضمام: |
edsdoj.329e364e593b470d835d8c6ce807099e |
| قاعدة البيانات: |
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