التفاصيل البيبلوغرافية
| العنوان: |
Synthesis and Characterization of Innovative Microgels Based on Polyacrylic Acid and Microalgae Cell Wall and Their Potential as Antigen Delivery Vehicles |
| المؤلفون: |
Ileana García-Silva, Miguel Olvera-Sosa, Benita Ortega-Berlanga, Víctor Ruíz-Rodríguez, Gabriela Palestino, Sergio Rosales-Mendoza |
| المصدر: |
Pharmaceutics, Vol 15, Iss 1, p 133 (2022) |
| بيانات النشر: |
MDPI AG, 2022. |
| سنة النشر: |
2022 |
| المجموعة: |
LCC:Pharmacy and materia medica |
| مصطلحات موضوعية: |
antigen carrier, mucosal immunization, hybrid polymer, inverse emulsion, Pharmacy and materia medica, RS1-441 |
| الوصف: |
In this study, hybrid polyacrylic acid and Schizochytrium sp. microalgae (PAA/Schizo) microgels were synthesized by inverse emulsion assisted by ultrasound using the cell wall fraction as crosslinker. Physicochemical characterization of PAA/Schizo microgels revealed polymeric spherical particles (288 ± 39 nm) and were deemed stable and negatively charged. The produced microgels are not inherently toxic as cell viability was sustained above 80% when mice splenocytes were exposed to concentrations ranging 10–900 µg/mL. PAA/Schizo microgels were evaluated as antigen delivery nanovehicle by adsorbing bovine serum albumin (BSA); with a loading efficiency of 72% and loading capacity of 362 µg/mg. Overall, intranasally-immunized BALB/c mice showed null IgG or IgA responses against PAA/Schizo microgel-BSA, whereas soluble BSA induced significant humoral responses in systemic and mucosal compartments. Splenocytes proliferation assay upon BSA stimulus revealed positive CD4+ T cells-proliferation response in PAA/Schizo microgels-BSA group. Thus, PAA/Schizo microgels constitute functional antigen delivery vehicles of simple and ecofriendly synthesis. Moreover, the use of cell wall fraction as cross-linker agent provides an alternative use for the generation of high-value products using residual algae biomass from the oil industry. Our data suggests that the PAA/Schizo microgels are potential antigen delivery vehicles for immunotherapy development. |
| نوع الوثيقة: |
article |
| وصف الملف: |
electronic resource |
| اللغة: |
English |
| تدمد: |
1999-4923 |
| Relation: |
https://www.mdpi.com/1999-4923/15/1/133; https://doaj.org/toc/1999-4923 |
| DOI: |
10.3390/pharmaceutics15010133 |
| URL الوصول: |
https://doaj.org/article/d329b6f9ffc148899c89d8f5b07f6901 |
| رقم الانضمام: |
edsdoj.329b6f9ffc148899c89d8f5b07f6901 |
| قاعدة البيانات: |
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