Academic Journal
Expression of Hemagglutinin–Neuraminidase and fusion epitopes of Newcastle Disease Virus in transgenic tobacco
| العنوان: | Expression of Hemagglutinin–Neuraminidase and fusion epitopes of Newcastle Disease Virus in transgenic tobacco |
|---|---|
| المؤلفون: | Amir Ghaffar Shahriari, Abdolreza Bagheri, Mohammad Reza Bassami, Saeid Malekzadeh-Shafaroudi, Alireza Afsharifar, Ali Niazi |
| المصدر: | Electronic Journal of Biotechnology, Vol 22, Iss C, Pp 38-43 (2016) |
| بيانات النشر: | Elsevier, 2016. |
| سنة النشر: | 2016 |
| المجموعة: | LCC:Biotechnology LCC:Biology (General) |
| مصطلحات موضوعية: | Agrobacterium tumefaciens, ELISA, Recombinant vaccine, Transgenic plants, Biotechnology, TP248.13-248.65, Biology (General), QH301-705.5 |
| الوصف: | Background: Newcastle disease is an important avian infectious disease that brings about vast economic damage for poultry industry. Transgenic plants represent a cost-effective system for the production of therapeutic proteins and are widely used for the production of poultry vaccines. In an attempt to develop a recombinant vaccine, a plant expression binary vector pBI121, containing the genes encoding Hemagglutinin–Neuraminidase (HN) and Fusion (F) epitopes of Newcastle Disease Virus (NDV) under the control of CaMV35S promoter and NOS terminator was constructed and introduced into the tobacco (Nicotiana tabacum) plant by Agrobacterium-mediated transformation. Results: Putative transgenic plants were screened in a selection medium containing 50 mg/L kanamycin and 30 mg/L meropenem. Integration of the foreign gene in plant genome was confirmed by PCR. Expression of foreign gene was analyzed at transcription level by RT-PCR and at translation level by means of dot blotting and ELISA. All analyses confirmed the expression of recombinant protein. Conclusion: Developments in genetic engineering have led to plant-based systems for recombinant vaccine production. In this research, tobacco plant was used to express F and HN epitopes of NDV. Our results indicate that for the production of recombinant vaccine, it is a novel strategy to use concatenated epitopes without their genetic fusion onto larger scaffold structure such as viral coat protein. |
| نوع الوثيقة: | article |
| وصف الملف: | electronic resource |
| اللغة: | English |
| تدمد: | 0717-3458 |
| Relation: | http://www.sciencedirect.com/science/article/pii/S0717345816300379; https://doaj.org/toc/0717-3458 |
| DOI: | 10.1016/j.ejbt.2016.05.003 |
| URL الوصول: | https://doaj.org/article/ee31ca33b953442bb9e79fd91cb372ea |
| رقم الانضمام: | edsdoj.31ca33b953442bb9e79fd91cb372ea |
| قاعدة البيانات: | Directory of Open Access Journals |
Full Text Finder | تدمد: | 07173458 |
|---|---|
| DOI: | 10.1016/j.ejbt.2016.05.003 |