التفاصيل البيبلوغرافية
| العنوان: |
Comparison of the sequences and expression levels of genes related to follicular development and atresia between prolific and nonprolific goat breeds |
| المؤلفون: |
Xiang‐Dong Zi, Liang Hu, Jian‐Yuan Lu, Shuang Liu, Yu‐Cai Zheng |
| المصدر: |
Veterinary Medicine and Science, Vol 6, Iss 2, Pp 187-195 (2020) |
| بيانات النشر: |
Wiley, 2020. |
| سنة النشر: |
2020 |
| المجموعة: |
LCC:Veterinary medicine |
| مصطلحات موضوعية: |
cloning, expression, goats, hormonal concentration, prolificacy, Veterinary medicine, SF600-1100 |
| الوصف: |
Abstract This study investigated the variations of the nucleotide sequences and ovarian expression levels of genes related to follicular development and atresia in prolific Jintang black goats and nonprolific Tibetan goats. Eight genes, FSHB, LHB, FSHR, LHCGR, ESR2, B4GANT2, BCL2 and BAX, were examined using reverse transcription‐polymerase chain reaction and quantitative real‐time PCR. The results showed that the nucleotide and deduced amino acid sequences of the LHB and BAX genes were not different, but there was one base change in the FSHR genes between the two breeds. There was one base change in the FSHB gene, which resulted in one amino acid substitution; there were nine base changes in the LHCGR gene, which resulted in five amino acid substitutions; and there were six base changes in the B4GANT2 gene, which resulted in four amino acid substitutions. The expression levels of the FSHR, LHCGR, ESR2, B4GANT2, BCL2 and BAX genes in the ovaries were not different between the two breeds. The plasma concentrations of FSH were not different, but the plasma concentrations of LH, P4 and E2 were lower in prolific Jintang black goats than in nonprolific Tibetan goats (P ˂ 0.05) at 40 hr after removal of the Controlled Internal Drug Release Devices. These results provide some foundations elucidating the endocrine and molecular mechanisms controlling ovulation rate in goats, but these need to be further verified. |
| نوع الوثيقة: |
article |
| وصف الملف: |
electronic resource |
| اللغة: |
English |
| تدمد: |
2053-1095 |
| Relation: |
https://doaj.org/toc/2053-1095 |
| DOI: |
10.1002/vms3.225 |
| URL الوصول: |
https://doaj.org/article/c301f00cedb7475bb4c677f623b081f6 |
| رقم الانضمام: |
edsdoj.301f00cedb7475bb4c677f623b081f6 |
| قاعدة البيانات: |
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