التفاصيل البيبلوغرافية
| العنوان: |
Nucleic Acid-Based Lateral Flow Biosensor for Salmonella Typhi and Salmonella Paratyphi: A Detection in Stool Samples of Suspected Carriers |
| المؤلفون: |
Zulkiply Nor Amalina, Muhammad Fazli Khalid, Sjafri Faizul Rahman, Muhamad Nuramin Ahmad, Mohamad Ahmad Najib, Asma Ismail, Ismail Aziah |
| المصدر: |
Diagnostics, Vol 11, Iss 4, p 700 (2021) |
| بيانات النشر: |
MDPI AG, 2021. |
| سنة النشر: |
2021 |
| المجموعة: |
LCC:Medicine (General) |
| مصطلحات موضوعية: |
multiplex PCR-lateral flow biosensor, point-of-care testing, Salmonella Typhi, Salmonella Paratyphi A, food handlers, typhoid carriers, Medicine (General), R5-920 |
| الوصف: |
A multiplex rapid detection system, based on a PCR-lateral flow biosensor (mPCR-LFB) was developed to identify Salmonella Typhi and Salmonella Paratyphi A from suspected carriers. The lower detection limit for S. Typhi and S. Paratyphi A was 0.16 and 0.08 ng DNA equivalent to 10 and 102 CFU/mL, respectively. Lateral flow biosensor was used for visual detection of mPCR amplicons (stgA, SPAint, ompC, internal amplification control) by labeling forward primers with fluorescein-isothiocyanate (FITC), Texas Red, dinitrophenol (DNP) and digoxigenin (DIG) and reverse primers with biotin. Binding of streptavidin-colloidal gold conjugate with the amplicons resulted in formation of a red color dots on the strip after 15–20 min of sample exposure. The nucleic acid lateral flow analysis of the mPCR-LFB was better in sensitivity and more rapid than the conventional agarose gel electrophoresis. Moreover, the mPCR-LFB showed 100% sensitivity and specificity when evaluated with stools spiked with 100 isolates of Salmonella genus and other bacteria. A prospective cohort study on stool samples of 1176 food handlers in outbreak areas (suspected carriers) resulted in 23 (2%) positive for S. Typhi. The developed assay has potential to be used for rapid detection of typhoid carriers in surveillance program. |
| نوع الوثيقة: |
article |
| وصف الملف: |
electronic resource |
| اللغة: |
English |
| تدمد: |
2075-4418 |
| Relation: |
https://www.mdpi.com/2075-4418/11/4/700; https://doaj.org/toc/2075-4418 |
| DOI: |
10.3390/diagnostics11040700 |
| URL الوصول: |
https://doaj.org/article/2ffe0a32fac44ad8b214fabfffa91a88 |
| رقم الانضمام: |
edsdoj.2ffe0a32fac44ad8b214fabfffa91a88 |
| قاعدة البيانات: |
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