التفاصيل البيبلوغرافية
| العنوان: |
Shedding light on biochemical features and potential immunogenic epitopes of Neospora caninum SAG1: In silico study |
| المؤلفون: |
Morteza Shams, Sasan Khazaei, Naser Nazari, Hamidreza Majidiani, Bahareh Kordi |
| المصدر: |
Informatics in Medicine Unlocked, Vol 27, Iss , Pp 100785- (2021) |
| بيانات النشر: |
Elsevier, 2021. |
| سنة النشر: |
2021 |
| المجموعة: |
LCC:Computer applications to medicine. Medical informatics |
| مصطلحات موضوعية: |
N. caninum, SAG1, Bioinformatics, Immunogenic epitopes, Computer applications to medicine. Medical informatics, R858-859.7 |
| الوصف: |
Vaccination is the only feasible way for appropriate prevention of Neospora caninum infection. The present in silico study was done to evaluate the physico-chemical properties and determine immunogenic epitopes of N. caninum SAG1 protein as a possible vaccine candidate. Web-based tools were used to predict physico-chemical properties, antigenicity, allergenicity, solubility, post-translational modification (PTM) sites, transmembrane domains and signal peptide, secondary and tertiary structures as well as intrinsically disordered regions, followed by identification and screening of potential linear and conformational B-cell epitopes and those peptides having affinity to bind mouse major histocompatibility complex (MHC) and cytotoxic T lymphocyte (CTL). The protein was stable in a test tube, had 319 residues with a molecular weight of 33.07 kDa, representing aliphatic index of 76.68 (thermotolerant) and GRAVY score of 0.031 (hydrophobic). There were 42 PTM sites and an N-terminally-located signal peptide in the sequence. Secondary structure comprised mostly by random coils, followed by strands and helices. Ramachandran plot of the refined model showed 71.7%, 24.9%, 3.0% and 0.4% residues in the favored, additional allowed, generously allowed and disallowed regions, correspondingly. Additionally, various potential B-cell (linear and conformational), CTL and MHC-binding epitopes were predicted for N. caninum SAG1. The findings of the present in silico study are a premise for vaccination strategies against neosporosis. |
| نوع الوثيقة: |
article |
| وصف الملف: |
electronic resource |
| اللغة: |
English |
| تدمد: |
2352-9148 |
| Relation: |
http://www.sciencedirect.com/science/article/pii/S2352914821002562; https://doaj.org/toc/2352-9148 |
| DOI: |
10.1016/j.imu.2021.100785 |
| URL الوصول: |
https://doaj.org/article/2fb02eb23863451993c1d4190799dab4 |
| رقم الانضمام: |
edsdoj.2fb02eb23863451993c1d4190799dab4 |
| قاعدة البيانات: |
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