التفاصيل البيبلوغرافية
| العنوان: |
The Effect of microRNA on the Production of Recombinant Protein in CHO Cells and its Mechanism |
| المؤلفون: |
Hui-Ning Liu, Wei-Hua Dong, Yan Lin, Zhao-Hui Zhang, Tian-Yun Wang |
| المصدر: |
Frontiers in Bioengineering and Biotechnology, Vol 10 (2022) |
| بيانات النشر: |
Frontiers Media S.A., 2022. |
| سنة النشر: |
2022 |
| المجموعة: |
LCC:Biotechnology |
| مصطلحات موضوعية: |
CHO cells, miRNA, recombinant protein, cell engineering, protein quality, Biotechnology, TP248.13-248.65 |
| الوصف: |
Recombinant protein production by mammalian cells is the initial step in the manufacture of many therapeutic proteins. Chinese hamster ovary (CHO) cells are the most common host system to produce recombinant therapeutic proteins (RTPs). However, it is still challenging to maintain high productivity ensuring the good quality of RTPs produced by CHO cells. MicroRNAs(miRNAs) are short regulatory non-coding RNAs that can regulate cellular behavior and complex phenotypes. It has been found that miRNAs can enhance the expression level of recombinant proteins in CHO cells by promoting proliferation, resisting apoptosis, and regulating metabolism. miRNAs also can affect the quality of RTPs. In this review, we will discuss the effect and mechanism of miRNA on the expression level and quality of recombinant proteins in CHO cells. |
| نوع الوثيقة: |
article |
| وصف الملف: |
electronic resource |
| اللغة: |
English |
| تدمد: |
2296-4185 |
| Relation: |
https://www.frontiersin.org/articles/10.3389/fbioe.2022.832065/full; https://doaj.org/toc/2296-4185 |
| DOI: |
10.3389/fbioe.2022.832065 |
| URL الوصول: |
https://doaj.org/article/2f9a3a2d30c74767947bc2f8c93d8c69 |
| رقم الانضمام: |
edsdoj.2f9a3a2d30c74767947bc2f8c93d8c69 |
| قاعدة البيانات: |
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