Academic Journal
Qualitative analysis of modified advanced-platelet-rich fibrin buffy coat among diabetic patients and tobacco smokers with chronic periodontitis: A cell block cytology study
| العنوان: | Qualitative analysis of modified advanced-platelet-rich fibrin buffy coat among diabetic patients and tobacco smokers with chronic periodontitis: A cell block cytology study |
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| المؤلفون: | Neelam Das, B J Janardhana Amaranath |
| المصدر: | Contemporary Clinical Dentistry, Vol 13, Iss 2, Pp 173-182 (2022) |
| بيانات النشر: | Wolters Kluwer Medknow Publications, 2022. |
| سنة النشر: | 2022 |
| المجموعة: | LCC:Dentistry |
| مصطلحات موضوعية: | fibrin network patterns, leukocyte concentration, light microscopy, modified (advanced platelet-rich fibrin), platelet aggregation, Dentistry, RK1-715 |
| الوصف: | Aim: The aim of this study was to evaluate and compare histologically the pattern of distribution of platelet and leukocyte concentration, quality of fibrin network, and the aggregation of platelets in the three zones of modified advanced platelet-rich fibrin (A-PRF) buffy coat among uncontrolled type 2 diabetic patients, tobacco smokers, and healthy individuals with chronic periodontitis. Materials and Methods: In this cross-sectional cytology study, 180 generalized chronic periodontitis patients (46–55 years) were enrolled – Group 1 (control group): 60 systemically healthy participants, Group 2 (test group): 60 heavy tobacco smokers, and Group 3 (test group): 60 uncontrolled type 2 diabetic patients. Fifteen milliliters of blood was drawn from all study participants. Modified A- PRF membrane was prepared and then processed histologically. Results: The distribution pattern of platelet and leukocyte concentration in modified (A-PRF) gradually declines from the serum to the red blood cell (RBC) end of a clot in all groups. We have assessed that the serum and middle end of modified (A-PRF) membrane had an increasingly moderate distribution of platelets and leukocytes in both type 2 diabetics and tobacco smokers. RBC end had more of sparse distribution in all the three groups. Healthy individuals exhibited 95% of reversible pattern, whereas tobacco smokers had 78.33% and uncontrolled type 2 diabetic patients had 93.33% of irreversible aggregation pattern of platelets. Loose fibrin network pattern was seen in all the three groups. These observations conclude that tobacco smokers had a high percentage of loose fibrin network with sparse distribution of cells. Males showed more loose fibrin network pattern of modified (A-PRF) membrane than compared to females. Conclusion: In the present study, it can be concluded that the application of modified (A-PRF) may provide enhanced periodontal healing in uncontrolled type 2 diabetic patients and tobacco smokers; furthermore, females may have better regenerative capacity compared to males. |
| نوع الوثيقة: | article |
| وصف الملف: | electronic resource |
| اللغة: | English |
| تدمد: | 0976-237X 0976-2361 |
| Relation: | http://www.contempclindent.org/article.asp?issn=0976-237X;year=2022;volume=13;issue=2;spage=173;epage=182;aulast=Das; https://doaj.org/toc/0976-237X; https://doaj.org/toc/0976-2361 |
| DOI: | 10.4103/ccd.ccd_1018_20 |
| URL الوصول: | https://doaj.org/article/2e7b79b594e54e54965ae8b9e1e27fa2 |
| رقم الانضمام: | edsdoj.2e7b79b594e54e54965ae8b9e1e27fa2 |
| قاعدة البيانات: | Directory of Open Access Journals |
Full Text Finder | تدمد: | 0976237X 09762361 |
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| DOI: | 10.4103/ccd.ccd_1018_20 |