التفاصيل البيبلوغرافية
| العنوان: |
Chemoradiation triggers antitumor Th1 and tissue resident memory-polarized immune responses to improve immune checkpoint inhibitors therapy |
| المؤلفون: |
Florence Marliot, Marc Van den Eynde, Franck Pagès, Romain Boidot, Céline Mirjolet, Amos Kirilovsky, Carine El Sissy, Nacilla Haicheur-Adjouri, Viorel Scripcariu, Olivier Adotevi, Laurie Spehner, Elodie Lauret Marie Joseph, Benoît Lecoester, Laura Boullerot, Laurie Rangan, Amélie Marguier, Florent Tochet, Magalie Dosset, Jihane Boustani, Patrice Ravel, Jean-René Pallandre, Francis Bonnefoy, Emmanuel Cornillot |
| المصدر: |
Journal for ImmunoTherapy of Cancer, Vol 9, Iss 7 (2021) |
| بيانات النشر: |
BMJ Publishing Group, 2021. |
| سنة النشر: |
2021 |
| المجموعة: |
LCC:Neoplasms. Tumors. Oncology. Including cancer and carcinogens |
| مصطلحات موضوعية: |
Neoplasms. Tumors. Oncology. Including cancer and carcinogens, RC254-282 |
| الوصف: |
Background Multiple synergistic combination approaches with cancer drugs are developed to overcome primary resistance to immunotherapy; however, the mechanistic rationale to combine chemoradiotherapy (CRT) with immune checkpoint inhibitors remains elusive.Methods This study described the immunological landscape of tumor microenvironment (TME) exposed to CRT. Tumor samples from patients with rectal cancer (n=43) treated with neoadjuvant CRT or radiotherapy were analyzed by nanostring and immunohistochemistry. Studies in mice were performed using three syngeneic tumors (TC1, CT26 and MC38). Tumor-bearing mice were treated either with platinum-based CRT, radiotherapy or chemotherapy. Anti-CTLA-4 and/or anti-Programmed Cell Death Receptor-1 (PD-1) therapy was used in combination with CRT. The therapy-exposed TME was screened by RNA sequencing and flow cytometry and tumor-infiltrating T lymphocyte functionality was evaluated by interferon (IFN)-γ ELIspot and intracellular cytokine staining.Results Front-to-front comparison analysis revealed the synergistic effect of CRT to establish a highly inflamed and Th1-polarized immune signature in the TME of patients and mice. In both settings, CRT-exposed TMEs were highly enriched in newly-infiltrated tumor-specific CD8+ T cells as well as tissue resident memory CD103+CD8+ T cells. In mice, CD8 T cells were involved in the antitumor response mediated by CRT and were primed by CRT-activated CD103+ dendritic cells. In the three tumor models, we showed that concurrent combination of CRT with a dual CTLA-4 and PD-1 blockade was required to achieve an optimal antitumor effect and to establish a broad and long-lasting protective antitumor T cell immunity.Conclusions Our results highlight the ability of CRT to stimulate strong antitumor T-cell-mediated immunity and tissue resident memory T activation in TME, to foster immune checkpoint inhibitors action. These findings have implications in clinic for the design clinical trials combining chemoradiation with immunotherapy. |
| نوع الوثيقة: |
article |
| وصف الملف: |
electronic resource |
| اللغة: |
English |
| تدمد: |
2051-1426 |
| Relation: |
https://jitc.bmj.com/content/9/7/e002256.full; https://doaj.org/toc/2051-1426 |
| DOI: |
10.1136/jitc-2020-002256 |
| URL الوصول: |
https://doaj.org/article/2c4df4d5a35b4af1b8a7572b3ea26484 |
| رقم الانضمام: |
edsdoj.2c4df4d5a35b4af1b8a7572b3ea26484 |
| قاعدة البيانات: |
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