Academic Journal
Unveiling the microbiome during post-partum uterine infection: a deep shotgun sequencing approach to characterize the dairy cow uterine microbiome
| العنوان: | Unveiling the microbiome during post-partum uterine infection: a deep shotgun sequencing approach to characterize the dairy cow uterine microbiome |
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| المؤلفون: | Carl Basbas, Adriana Garzon, Cory Schlesener, Machteld van Heule, Rodrigo Profeta, Bart C. Weimer, Noelia Silva-del-Rio, Barbara A. Byrne, Betsy Karle, Sharif S. Aly, Fabio S. Lima, Richard V. Pereira |
| المصدر: | Animal Microbiome, Vol 5, Iss 1, Pp 1-18 (2023) |
| بيانات النشر: | BMC, 2023. |
| سنة النشر: | 2023 |
| المجموعة: | LCC:Veterinary medicine LCC:Microbiology |
| مصطلحات موضوعية: | Uterine microbiome, Microbiota, Metritis, Diversity, Veterinary medicine, SF600-1100, Microbiology, QR1-502 |
| الوصف: | Abstract Background The goal of this study was to assess the microbial ecology and diversity present in the uterus of post-partum dairy cows with and without metritis from 24 commercial California dairy farms using shotgun metagenomics. A set subset of 95 intrauterine swab samples, taken from a larger selection of 307 individual cow samples previously collected, were examined for α and β diversity and differential abundance associated with metritis. Cows within 21 days post-partum were categorized into one of three clinical groups during sample collection: control (CT, n = 32), defined as cows with either no vaginal discharge or a clear, non-purulent mucus vaginal discharge; metritis (MET, n = 33), defined as a cow with watery, red or brown colored, and fetid vaginal discharge; and purulent discharge cows (PUS, n = 31), defined as a non-fetid purulent or mucopurulent vaginal discharge. Results All three clinical groups (CT, MET, and PUS) were highly diverse, with the top 12 most abundant genera accounting for 10.3%, 8.8%, and 10.1% of mean relative abundance, respectively. The α diversity indices revealed a lower diversity from samples collected from MET and PUS when compared to CT cows. PERMANOVA statistical testing revealed a significant difference (P adjusted |
| نوع الوثيقة: | article |
| وصف الملف: | electronic resource |
| اللغة: | English |
| تدمد: | 2524-4671 |
| Relation: | https://doaj.org/toc/2524-4671 |
| DOI: | 10.1186/s42523-023-00281-5 |
| URL الوصول: | https://doaj.org/article/2b766c4592554de4b6c5f39180add0dc |
| رقم الانضمام: | edsdoj.2b766c4592554de4b6c5f39180add0dc |
| قاعدة البيانات: | Directory of Open Access Journals |
Full Text Finder | تدمد: | 25244671 |
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| DOI: | 10.1186/s42523-023-00281-5 |