Academic Journal
The D113N mutation in the RING E3 ubiquitin protein ligase gene is not associated with ex vivo susceptibility to common anti-malarial drugs in African Plasmodium falciparum isolates
| العنوان: | The D113N mutation in the RING E3 ubiquitin protein ligase gene is not associated with ex vivo susceptibility to common anti-malarial drugs in African Plasmodium falciparum isolates |
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| المؤلفون: | Mathieu Gendrot, Francis Tsombeng Foguim, Marie Gladys Robert, Rémy Amalvict, Joel Mosnier, Nicolas Benoit, Marylin Madamet, Bruno Pradines, the French National Reference Centre for Imported Malaria Study Group |
| المصدر: | Malaria Journal, Vol 17, Iss 1, Pp 1-8 (2018) |
| بيانات النشر: | BMC, 2018. |
| سنة النشر: | 2018 |
| المجموعة: | LCC:Arctic medicine. Tropical medicine LCC:Infectious and parasitic diseases |
| مصطلحات موضوعية: | Malaria, Plasmodium falciparum, Anti-malarial drug, In vitro, Resistance, Molecular marker, Arctic medicine. Tropical medicine, RC955-962, Infectious and parasitic diseases, RC109-216 |
| الوصف: | Abstract Background Plasmodium falciparum resistance to artemisinin-based combination therapy has emerged and spread in Southeast Asia. In areas where artemisinin resistance is emerging, the efficacy of combination is now based on partner drugs. In this context, the identification of novel markers of resistance is essential to monitor the emergence and spread of resistance to these partner drugs. The ubiquitylation pathway could be a possible target for anti-malarial compounds and might be involved in resistance. Polymorphisms in the E3 ubiquitin-protein ligase (PF3D7_0627300) gene could be associated with decreased in vitro susceptibility to anti-malarial drugs. Methods Plasmodium falciparum isolates were collected from patients hospitalized in France with imported malaria from a malaria-endemic country from January 2015 to December 2016 and, more particularly, from African French-speaking countries. In total, 215 isolates were successfully sequenced for the E3 ubiquitin-protein ligase gene and assessed for ex vivo susceptibility to anti-malarial drugs. Results The D113N mutation in the RING E3 ubiquitin-protein ligase gene was present in 147 out of the 215 samples (68.4%). The IC50 values for the ten anti-malarial drugs were not significantly different between the wild-type and mutant parasites (p values between 0.225 and 0.933). There was no significant difference in terms of the percentage of parasites with decreased susceptibility between the D113 wild-type and the 133N mutated P. falciparum strains (p values between 0.541 and 1). Conclusion The present data confirmed the absence of the association between polymorphisms in the RING E3 ubiquitin-protein ligase gene and the ex vivo susceptibility to common anti-malarial drugs in African P. falciparum isolates. |
| نوع الوثيقة: | article |
| وصف الملف: | electronic resource |
| اللغة: | English |
| تدمد: | 1475-2875 |
| Relation: | http://link.springer.com/article/10.1186/s12936-018-2252-2; https://doaj.org/toc/1475-2875 |
| DOI: | 10.1186/s12936-018-2252-2 |
| URL الوصول: | https://doaj.org/article/2b4d74cc3c6c4ddcaf77eb24862fef63 |
| رقم الانضمام: | edsdoj.2b4d74cc3c6c4ddcaf77eb24862fef63 |
| قاعدة البيانات: | Directory of Open Access Journals |
Full Text Finder | تدمد: | 14752875 |
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| DOI: | 10.1186/s12936-018-2252-2 |