Academic Journal
Vascular endothelial growth factor inhibits dendritic cell maturation
| العنوان: | Vascular endothelial growth factor inhibits dendritic cell maturation |
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| المؤلفون: | Luciana Cavalheiro Marti, Patricia Renovato Tobo, Ana Carolina Souza Ramos de Carvalho, Alipio Oliveira do Carmo, Oswaldo Keith Okamoto, Carlos Alberto Moreira-Filho |
| المصدر: | Einstein (São Paulo), Vol 3, Iss 1, Pp 14-18 (2005) |
| بيانات النشر: | Instituto Israelita de Ensino e Pesquisa Albert Einstein, 2005. |
| سنة النشر: | 2005 |
| المجموعة: | LCC:Medicine |
| مصطلحات موضوعية: | Vascular endothelial growth factor A, Dendritic cells, Immune system, Medicine |
| الوصف: | Objective: In this paper we investigated the effects of vascularendothelial growth factor on dendritic cells differentiation andmaturation from monocytic precursors. Methods: CD14+/CD34-progenitor cells were obtained from umbilical cord blood, purifiedby magnetic cell sorting, and cultivated with IL-4 and GM-CSF, inthe presence or absence of vascular endothelial growth factor.Maturation of dendritic cells was induced after six days of cultureby 24h-treatment with lipopolysaccharide (LPS). Results:Expression of marker proteins for immature (CD14 and DC-SIGN)and mature(CD83) dendritic cells was detected by fluorescencemicroscopy and flow cytometry using monoclonal antibodies(mAb). Under proper differentiating conditions, treatment withvascular endothelial growth factor did not change the expressionof immature DC markers. Following maturation with LPS, increasedamount of CD14+ and DC-SIGN+ cells and decreased CD83+ cellpopulation were detected in vascular endothelial growth factortreatedcultures. Conclusions: Our data suggest that vascularendothelial growth factor does not affect the differentiation ofCD14+/CD34- progenitor cells into immature dendritic cells, butit reduces the efficiency of dendritic cells maturation in vitro. Thisvascular endothelial growth factor-mediated effect on dendriticcells function may influence anti-tumor immune responses. |
| نوع الوثيقة: | article |
| وصف الملف: | electronic resource |
| اللغة: | English Portuguese |
| تدمد: | 1679-4508 |
| Relation: | http://www.einstein.br/revista/biblioteca/artigos/vol3/num_1/Vol3_%20N1_P14.pdf; https://doaj.org/toc/1679-4508 |
| URL الوصول: | https://doaj.org/article/29c2f8444f0e4d61b245b8ddb0963995 |
| رقم الانضمام: | edsdoj.29c2f8444f0e4d61b245b8ddb0963995 |
| قاعدة البيانات: | Directory of Open Access Journals |
| تدمد: | 16794508 |
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