Academic Journal
Tag-mediated single-step purification and immobilization of recombinant proteins toward protein-engineered advanced materials
| العنوان: | Tag-mediated single-step purification and immobilization of recombinant proteins toward protein-engineered advanced materials |
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| المؤلفون: | Ana I. Freitas, Lucília Domingues, Tatiana Q. Aguiar |
| المصدر: | Journal of Advanced Research, Vol 36, Iss , Pp 249-264 (2022) |
| بيانات النشر: | Elsevier, 2022. |
| سنة النشر: | 2022 |
| المجموعة: | LCC:Medicine (General) LCC:Science (General) |
| مصطلحات موضوعية: | Protein tags, Solid-binding peptides, Non-covalent binding, Covalent binding, Protein-engineered materials, Protein purification/immobilization, Medicine (General), R5-920, Science (General), Q1-390 |
| الوصف: | Background: The potential applications of protein-engineered functional materials are so wide and exciting that the interest in these eco-friendly advanced materials will further expand in the future. Tag-mediated protein purification/immobilization technologies have emerged as green and cost-effective approaches for the fabrication of such materials. Strategies that combine the purification and immobilization of recombinant proteins/peptides onto/into natural, synthetic or hybrid materials in a single-step are arising and attracting increasing interest. Aim of Review: This review highlights the most significant advances of the last 5 years within the scope of tag-mediated protein purification/immobilization and elucidates their contributions for the development of efficient single-step purification and immobilization strategies. Recent progresses in the field of protein-engineered materials created using innovative protein-tag combinations and future opportunities created by these new technologies are also summarized and identified herein. Key Scientific Concepts of Review: Protein purification/immobilization tags present a remarkable ability to establish specific non-covalent/covalent interactions between solid materials and biological elements, which prompted the creation of tailor-made and advanced functional materials, and of next-generation hybrid materials. Affinity tags can bind to a wide range of materials (of synthetic, natural or hybrid nature), being most suitable for protein purification. Covalently binding tags are most suitable for long-term protein immobilization, but can only bind naturally to protein-based materials. Hybrid affinity-covalently binding tags have allowed efficient one-step purification and immobilization of proteins onto different materials, as well as the development of innovative protein-engineered materials. Self-aggregating tags have been particularly useful in combination with other tags for generating protein-engineered materials with self-assembling, flexible and/or responsive properties. While these tags have been mainly explored for independent protein purification, immobilization or functionalization purposes, efficient strategies that combine tag-mediated purification and immobilization/functionalization in a single-step will be essential to guarantee the sustainable manufacturing of advanced protein-engineered materials. |
| نوع الوثيقة: | article |
| وصف الملف: | electronic resource |
| اللغة: | English |
| تدمد: | 2090-1232 |
| Relation: | http://www.sciencedirect.com/science/article/pii/S2090123221001065; https://doaj.org/toc/2090-1232 |
| DOI: | 10.1016/j.jare.2021.06.010 |
| URL الوصول: | https://doaj.org/article/294afbda13e14442be3608d4c44d2d1d |
| رقم الانضمام: | edsdoj.294afbda13e14442be3608d4c44d2d1d |
| قاعدة البيانات: | Directory of Open Access Journals |
Full Text Finder | تدمد: | 20901232 |
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| DOI: | 10.1016/j.jare.2021.06.010 |