Academic Journal
Transcriptional profiling of sequentially generated septal neuron fates
| العنوان: | Transcriptional profiling of sequentially generated septal neuron fates |
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| المؤلفون: | Miguel Turrero García, Sarah K Stegmann, Tiara E Lacey, Christopher M Reid, Sinisa Hrvatin, Caleb Weinreb, Manal A Adam, M Aurel Nagy, Corey C Harwell |
| المصدر: | eLife, Vol 10 (2021) |
| بيانات النشر: | eLife Sciences Publications Ltd, 2021. |
| سنة النشر: | 2021 |
| المجموعة: | LCC:Medicine LCC:Science LCC:Biology (General) |
| مصطلحات موضوعية: | septal nucleus, neurogenesis, cell fate, transcription factors, Medicine, Science, Biology (General), QH301-705.5 |
| الوصف: | The septum is a ventral forebrain structure known to regulate innate behaviors. During embryonic development, septal neurons are produced in multiple proliferative areas from neural progenitors following transcriptional programs that are still largely unknown. Here, we use a combination of single-cell RNA sequencing, histology, and genetic models to address how septal neuron diversity is established during neurogenesis. We find that the transcriptional profiles of septal progenitors change along neurogenesis, coinciding with the generation of distinct neuron types. We characterize the septal eminence, an anatomically distinct and transient proliferative zone composed of progenitors with distinctive molecular profiles, proliferative capacity, and fate potential compared to the rostral septal progenitor zone. We show that Nkx2.1-expressing septal eminence progenitors give rise to neurons belonging to at least three morphological classes, born in temporal cohorts that are distributed across different septal nuclei in a sequential fountain-like pattern. Our study provides insight into the molecular programs that control the sequential production of different neuronal types in the septum, a structure with important roles in regulating mood and motivation. |
| نوع الوثيقة: | article |
| وصف الملف: | electronic resource |
| اللغة: | English |
| تدمد: | 2050-084X |
| Relation: | https://elifesciences.org/articles/71545; https://doaj.org/toc/2050-084X |
| DOI: | 10.7554/eLife.71545 |
| URL الوصول: | https://doaj.org/article/ea2869b1a4454656b939d2b3305dfe40 |
| رقم الانضمام: | edsdoj.2869b1a4454656b939d2b3305dfe40 |
| قاعدة البيانات: | Directory of Open Access Journals |
Full Text Finder | تدمد: | 2050084X |
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| DOI: | 10.7554/eLife.71545 |