التفاصيل البيبلوغرافية
| العنوان: |
Impact of age on the host response to sepsis in a murine model of fecal-induced peritonitis |
| المؤلفون: |
Neha Sharma, Alex Chen, Leah Heinen, Ruth Liu, Dhruva J. Dwivedi, Ji Zhou, Manoj M. Lalu, Asher A. Mendelson, Braedon McDonald, Colin A. Kretz, Alison E. Fox-Robichaud, Patricia C. Liaw |
| المصدر: |
Intensive Care Medicine Experimental, Vol 12, Iss 1, Pp 1-13 (2024) |
| بيانات النشر: |
SpringerOpen, 2024. |
| سنة النشر: |
2024 |
| المجموعة: |
LCC:Medical emergencies. Critical care. Intensive care. First aid |
| مصطلحات موضوعية: |
Sepsis, Age, Fecal-induced peritonitis model, Immunothrombosis, National Preclinical Sepsis Platform, Medical emergencies. Critical care. Intensive care. First aid, RC86-88.9 |
| الوصف: |
Abstract Introduction Despite older adults being more vulnerable to sepsis, most preclinical research on sepsis has been conducted using young animals. This results in decreased scientific validity since age is an independent predictor of poor outcome. In this study, we explored the impact of aging on the host response to sepsis using the fecal-induced peritonitis (FIP) model developed by the National Preclinical Sepsis Platform (NPSP). Methods C57BL/6 mice (3 or 12 months old) were injected intraperitoneally with rat fecal slurry (0.75 mg/g) or a control vehicle. To investigate the early stage of sepsis, mice were culled at 4 h, 8 h, or 12 h to investigate disease severity, immunothrombosis biomarkers, and organ injury. Mice received buprenorphine at 4 h post-FIP. A separate cohort of FIP mice were studied for 72 h (with buprenorphine given at 4 h, 12 h, and then every 12 h post-FIP and antibiotics/fluids starting at 12 h post-FIP). Organs were harvested, plasma levels of Interleukin (IL)-6, IL-10, monocyte chemoattract protein (MCP-1)/CCL2, thrombin-antithrombin (TAT) complexes, cell-free DNA (CFDNA), and ADAMTS13 activity were quantified, and bacterial loads were measured. Results In the 12 h time course study, aged FIP mice demonstrated increased inflammation and injury to the lungs compared to young FIP mice. In the 72 h study, aged FIP mice exhibited a higher mortality rate (89%) compared to young FIP mice (42%) (p |
| نوع الوثيقة: |
article |
| وصف الملف: |
electronic resource |
| اللغة: |
English |
| تدمد: |
2197-425X |
| Relation: |
https://doaj.org/toc/2197-425X |
| DOI: |
10.1186/s40635-024-00609-8 |
| URL الوصول: |
https://doaj.org/article/27fd2b1cf7f24f92a98ec9a101867c38 |
| رقم الانضمام: |
edsdoj.27fd2b1cf7f24f92a98ec9a101867c38 |
| قاعدة البيانات: |
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