Academic Journal
Topological Characterization of Human and Mouse m5C Epitranscriptome Revealed by Bisulfite Sequencing
| العنوان: | Topological Characterization of Human and Mouse m5C Epitranscriptome Revealed by Bisulfite Sequencing |
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| المؤلفون: | Zhen Wei, Subbarayalu Panneerdoss, Santosh Timilsina, Jingting Zhu, Tabrez A. Mohammad, Zhi-Liang Lu, João Pedro de Magalhães, Yidong Chen, Rong Rong, Yufei Huang, Manjeet K. Rao, Jia Meng |
| المصدر: | International Journal of Genomics, Vol 2018 (2018) |
| بيانات النشر: | Wiley, 2018. |
| سنة النشر: | 2018 |
| المجموعة: | LCC:Genetics |
| مصطلحات موضوعية: | Genetics, QH426-470 |
| الوصف: | Background. Compared with the well-studied 5-methylcytosine (m5C) in DNA, the role and topology of epitranscriptome m5C remain insufficiently characterized. Results. Through analyzing transcriptome-wide m5C distribution in human and mouse, we show that the m5C modification is significantly enriched at 5′ untranslated regions (5′UTRs) of mRNA in human and mouse. With a comparative analysis of the mRNA and DNA methylome, we demonstrate that, like DNA methylation, transcriptome m5C methylation exhibits a strong clustering effect. Surprisingly, an inverse correlation between mRNA and DNA m5C methylation is observed at CpG sites. Further analysis reveals that RNA m5C methylation level is positively correlated with both RNA expression and RNA half-life. We also observed that the methylation level of mitochondrial RNAs is significantly higher than RNAs transcribed from the nuclear genome. Conclusions. This study provides an in-depth topological characterization of transcriptome-wide m5C modification by associating RNA m5C methylation patterns with transcriptional expression, DNA methylations, RNA stabilities, and mitochondrial genome. |
| نوع الوثيقة: | article |
| وصف الملف: | electronic resource |
| اللغة: | English |
| تدمد: | 2314-436X 2314-4378 |
| Relation: | https://doaj.org/toc/2314-436X; https://doaj.org/toc/2314-4378 |
| DOI: | 10.1155/2018/1351964 |
| URL الوصول: | https://doaj.org/article/271b300dce334098acc2ce7c40c02218 |
| رقم الانضمام: | edsdoj.271b300dce334098acc2ce7c40c02218 |
| قاعدة البيانات: | Directory of Open Access Journals |
Full Text Finder | تدمد: | 2314436X 23144378 |
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| DOI: | 10.1155/2018/1351964 |