Academic Journal
The relationship between miRNA-26b and connective tissue growth factor in rat models of aortic banding and debanding
| العنوان: | The relationship between miRNA-26b and connective tissue growth factor in rat models of aortic banding and debanding |
|---|---|
| المؤلفون: | Jung Sun Cho, Jongho Lee, Ki Cheol Park, Keum-Jin Yang, Eun Joo Cho |
| المصدر: | The Korean Journal of Internal Medicine, Vol 36, Iss 3, Pp 596-607 (2021) |
| بيانات النشر: | The Korean Association of Internal Medicine, 2021. |
| سنة النشر: | 2021 |
| المجموعة: | LCC:Medicine |
| مصطلحات موضوعية: | fibrosis, aortic banding, aortic debanding, connective tissue growth factor, mirna-26b, Medicine |
| الوصف: | Background/Aims Connective tissue growth factor (CTGF) is a profibrotic factor implicated in pressure overload-mediated myocardial fibrosis. In this study, we determined the role of predicted CTGF-targeting microRNAs (miRNAs) in rat models of aortic stenosis and reverse cardiac remodeling. Methods Minimally invasive ascending aortic banding was performed in 24 7-week-old male Sprague-Dawley rats, which were divided into three groups. The banding group consisted of eight rats that were sacrificed immediately after 6 weeks of aortic constriction. The debanding group underwent aortic constriction for 4 weeks and was sacrificed 2 weeks after band removal. The third group underwent sham surgery. We investigated the expression of CTGF, transforming growth factor-β1 (TGFβ1), and matrix metalloproteinase-2 using ELISA and examined miRNA-26b, miRNA-133a, and miRNA-19b as predicted CTGF-targeting miRNAs based on miRNA databases in 24-hour TGFβ-stimulated and TGFβ- washed fibroblasts and myocardial tissues from all subjects. Results CTGF was elevated in 24-hour TGFβ-stimulated fibroblasts and decreased in 24-hour TGFβ-washed fibroblasts. miRNA-26b was significantly increased in TGFβ-washed fibroblasts compared with control and TGFβ-stimulated fibroblasts (p < 0.05). CTGF expression was significantly higher in the banding group than that in the sham and debanding groups. The relative expression levels of miRNA-26b were higher in the debanding group than in the banding group. Conclusions The results of our study using models of aortic banding and debanding suggested that miRNA-26b was significantly increased after aortic debanding. The in vitro model yielded the same results: miRNA-26b was upregulated after removal of TGFβ from fibroblasts. |
| نوع الوثيقة: | article |
| وصف الملف: | electronic resource |
| اللغة: | English |
| تدمد: | 1226-3303 2005-6648 |
| Relation: | http://www.kjim.org/upload/pdf/kjim-2019-120.pdf; https://doaj.org/toc/1226-3303; https://doaj.org/toc/2005-6648 |
| DOI: | 10.3904/kjim.2019.120 |
| URL الوصول: | https://doaj.org/article/26c4beb8807e409c86cc0a12ff00c2c6 |
| رقم الانضمام: | edsdoj.26c4beb8807e409c86cc0a12ff00c2c6 |
| قاعدة البيانات: | Directory of Open Access Journals |
Full Text Finder | تدمد: | 12263303 20056648 |
|---|---|
| DOI: | 10.3904/kjim.2019.120 |