التفاصيل البيبلوغرافية
| العنوان: |
Role of autophagy machinery dysregulation in bacterial chondronecrosis with osteomyelitis |
| المؤلفون: |
Alison Ramser, Elizabeth Greene, Adnan A.K. Alrubaye, Robert Wideman, Sami Dridi |
| المصدر: |
Poultry Science, Vol 101, Iss 5, Pp 101750- (2022) |
| بيانات النشر: |
Elsevier, 2022. |
| سنة النشر: |
2022 |
| المجموعة: |
LCC:Animal culture |
| مصطلحات موضوعية: |
autophagy, broiler, bacterial chondronecrosis with osteomyelitis, lameness, osteoblast, Animal culture, SF1-1100 |
| الوصف: |
ABSTRACT: Autophagy is a cell survival and homeostasis mechanism involving lysosomal degradation of cellular components and foreign bodies. It plays a role in bone homeostasis, skeletal diseases, and bacterial infections as both a cell-survival or cell-death pathway. This study sought to determine if autophagy played a role in bacterial chondronecrosis with osteomyelitis (BCO). BCO is a prominent cause of lameness in modern broilers and results from bacterial infection of mechanically stressed leg bone growth plates. The protein and gene expression of key autophagy machinery was analyzed in both normal and BCO-affected broilers using real-time qPCR and immunoblot, respectively. Gene expression showed a significant downregulation of key target signatures involved in every stage of autophagy in BCO-affected bone, such as ATG13, SQSTM1 (p62), ATG9B, ATG16L, ATG12, LC3C, and RAB7A. Additionally, protein expression for LC3 was also significantly lower in BCO. An in vitro study using human fetal osteoblast cells challenged with BCO isolate, Staphylococcus agnetis 908, showed a similar dysregulation of autophagy machinery along with a significant decrease in cell viability. When autophagy was inhibited via 3-methyladenine or chloroquine, comparable decreases in cell viability were seen along with dysregulation of autophagy machinery. Together, these results are the first to implicate autophagy machinery dysregulation in the pathology of BCO. |
| نوع الوثيقة: |
article |
| وصف الملف: |
electronic resource |
| اللغة: |
English |
| تدمد: |
0032-5791 |
| Relation: |
http://www.sciencedirect.com/science/article/pii/S0032579122000554; https://doaj.org/toc/0032-5791 |
| DOI: |
10.1016/j.psj.2022.101750 |
| URL الوصول: |
https://doaj.org/article/26b7d79e7716405682cb3208cc12f9b6 |
| رقم الانضمام: |
edsdoj.26b7d79e7716405682cb3208cc12f9b6 |
| قاعدة البيانات: |
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