التفاصيل البيبلوغرافية
| العنوان: |
BRD4 is involved in viral exacerbation of chronic obstructive pulmonary disease |
| المؤلفون: |
Yifei Duan, Siyi Zhou, Jianmiao Wang |
| المصدر: |
Respiratory Research, Vol 24, Iss 1, Pp 1-12 (2023) |
| بيانات النشر: |
BMC, 2023. |
| سنة النشر: |
2023 |
| المجموعة: |
LCC:Diseases of the respiratory system |
| مصطلحات موضوعية: |
Bromodomain protein 4 (BRD4), Chronic obstructive pulmonary disease, Exacerbation, Inflammation, Viral infection, Diseases of the respiratory system, RC705-779 |
| الوصف: |
Abstract Background Our previous studies have suggested that bromodomain protein 4 (BRD4) is increased in the lung of stable chronic obstructive pulmonary disease (COPD) patients, which has been shown to be involved in inflammatory responses. We investigated its role in the viral exacerbation of COPD. Methods BRD4, interleukin (IL)-6 and IL-8 were measured in the blood and sputum of stable COPD patients and patients with viral exacerbation. Mice were exposed to cigarette smoke (CS) and/or infected with influenza virus as an in vivo model. BRD4, IL-6 and keratinocyte-derived chemokine (KC) were measured in the lung. BEAS-2B cells were treated with CS extract and/or influenza virus as an in vitro model. BRD4, IL-6 and IL-8 were measured in the cells and/or culture supernatant. Results BRD4 was increased in COPD patients with viral exacerbation compared with those in stable condition and its expression was correlated with IL-6 and IL-8 expression. Inflammatory cells, IL-6, KC and BRD4 were synergistically induced in the lung of mice by viral infection and CS exposure, and the former three were decreased by JQ1 (BRD4 inhibitor) treatment. IL-6, IL-8 and BRD4 were significantly induced by CS extract and influenza virus in bronchial epithelial cells, and this upregulation was suppressed by knockdown of BRD4 expression. Conclusions Our findings indicate that CS and viruses may synergistically induce IL-6 and IL-8 expression through their synergistic induction of BRD4 expression, which might contribute to the enhanced inflammatory response in the viral exacerbation of COPD. |
| نوع الوثيقة: |
article |
| وصف الملف: |
electronic resource |
| اللغة: |
English |
| تدمد: |
1465-993X |
| Relation: |
https://doaj.org/toc/1465-993X |
| DOI: |
10.1186/s12931-023-02348-y |
| URL الوصول: |
https://doaj.org/article/26373cdbeb2e44e0be9f0ff7c0bf7495 |
| رقم الانضمام: |
edsdoj.26373cdbeb2e44e0be9f0ff7c0bf7495 |
| قاعدة البيانات: |
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