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Single gene analysis in yeast suggests nonequilibrium regulatory dynamics for transcription

التفاصيل البيبلوغرافية
العنوان: Single gene analysis in yeast suggests nonequilibrium regulatory dynamics for transcription
المؤلفون: Robert Shelansky, Sara Abrahamsson, Christopher R. Brown, Michael Doody, Tineke L. Lenstra, Daniel R. Larson, Hinrich Boeger
المصدر: Nature Communications, Vol 15, Iss 1, Pp 1-9 (2024)
بيانات النشر: Nature Portfolio, 2024.
سنة النشر: 2024
المجموعة: LCC:Science
مصطلحات موضوعية: Science
الوصف: Abstract Fluctuations in the initiation rate of transcription, the first step in gene expression, ensue from the stochastic behavior of the molecular process that controls transcription. In steady state, the regulatory process is often assumed to operate reversibly, i.e., in equilibrium. However, reversibility imposes fundamental limits to information processing. For instance, the assumption of equilibrium is difficult to square with the precision with which the regulatory process executes its task in eukaryotes. Here we provide evidence — from microscopic analyses of the transcription dynamics at a single gene copy of yeast — that the regulatory process for transcription is cyclic and irreversible (out of equilibrium). The necessary coupling to reservoirs of free energy occurs via sequence-specific transcriptional activators and the recruitment, in part, of ATP-dependent chromatin remodelers. Our findings may help explain how eukaryotic cells reconcile the dual but opposing requirements for fast regulatory kinetics and high regulatory specificity.
نوع الوثيقة: article
وصف الملف: electronic resource
اللغة: English
تدمد: 2041-1723
27506789
Relation: https://doaj.org/toc/2041-1723
DOI: 10.1038/s41467-024-50419-5
URL الوصول: https://doaj.org/article/2608996d29874af0999a2ab275067895
رقم الانضمام: edsdoj.2608996d29874af0999a2ab275067895
قاعدة البيانات: Directory of Open Access Journals
الوصف
تدمد:20411723
27506789
DOI:10.1038/s41467-024-50419-5