التفاصيل البيبلوغرافية
| العنوان: |
Structural and Mechanistic Analyses Reveal a Unique Cas4-like Protein in the Mimivirus Virophage Resistance Element System |
| المؤلفون: |
Chao Dou, Mingjing Yu, Yijun Gu, Jinjing Wang, Kun Yin, Chunlai Nie, Xiaofeng Zhu, Shiqian Qi, Yuquan Wei, Wei Cheng |
| المصدر: |
iScience, Vol 3, Iss , Pp 1-10 (2018) |
| بيانات النشر: |
Elsevier, 2018. |
| سنة النشر: |
2018 |
| المجموعة: |
LCC:Science |
| مصطلحات موضوعية: |
Science |
| الوصف: |
Summary: A clustered regularly interspaced short palindromic repeats (CRISPR)-like “mimivirus virophage resistance element” (MIMIVIRE) system, which contains specific cascade genes and a CRISPR array against virophages, was reported in mimiviruses. An essential component of the MIMIVIRE system is R354, encoding a nuclease and a likely functional homolog of Cas4. Here we show that R354 is a dual nuclease with both exonuclease and endonuclease activities. Structural analysis revealed that the catalytic core domain of R354 is similar to those of Cas4 and λ exonuclease despite their low sequence identity. R354 forms a homodimer that is important for its exonuclease but not endonuclease activity. Structural comparisons between the active and semi-active states of R354 demonstrated that an activation loop adjacent to the catalytic site is critical for enzymatic activity. Overall, the results suggest that R354 belongs to a novel MIMIVIRE system involved in innate virus immunity and provides a template for the identification of new CRISPR systems in other species. : Virology; Structural Biology; Protein Structure Aspects Subject Areas: Virology, Structural Biology, Protein Structure Aspects |
| نوع الوثيقة: |
article |
| وصف الملف: |
electronic resource |
| اللغة: |
English |
| تدمد: |
2589-0042 |
| Relation: |
http://www.sciencedirect.com/science/article/pii/S2589004218300336; https://doaj.org/toc/2589-0042 |
| DOI: |
10.1016/j.isci.2018.04.001 |
| URL الوصول: |
https://doaj.org/article/25624fde35f84394801bd4825cad2966 |
| رقم الانضمام: |
edsdoj.25624fde35f84394801bd4825cad2966 |
| قاعدة البيانات: |
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