Academic Journal
Ribosome collisions trigger cis-acting feedback inhibition of translation initiation
| العنوان: | Ribosome collisions trigger cis-acting feedback inhibition of translation initiation |
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| المؤلفون: | Szymon Juszkiewicz, Greg Slodkowicz, Zhewang Lin, Paula Freire-Pritchett, Sew-Yeu Peak-Chew, Ramanujan S Hegde |
| المصدر: | eLife, Vol 9 (2020) |
| بيانات النشر: | eLife Sciences Publications Ltd, 2020. |
| سنة النشر: | 2020 |
| المجموعة: | LCC:Medicine LCC:Science LCC:Biology (General) |
| مصطلحات موضوعية: | translation, ribosome, quality control, Medicine, Science, Biology (General), QH301-705.5 |
| الوصف: | Translation of aberrant mRNAs can cause ribosomes to stall, leading to collisions with trailing ribosomes. Collided ribosomes are specifically recognised by ZNF598 to initiate protein and mRNA quality control pathways. Here we found using quantitative proteomics of collided ribosomes that EDF1 is a ZNF598-independent sensor of ribosome collisions. EDF1 stabilises GIGYF2 at collisions to inhibit translation initiation in cis via 4EHP. The GIGYF2 axis acts independently of the ZNF598 axis, but each pathway’s output is more pronounced without the other. We propose that the widely conserved and highly abundant EDF1 monitors the transcriptome for excessive ribosome density, then triggers a GIGYF2-mediated response to locally and temporarily reduce ribosome loading. Only when collisions persist is translation abandoned to initiate ZNF598-dependent quality control. This tiered response to ribosome collisions would allow cells to dynamically tune translation rates while ensuring fidelity of the resulting protein products. |
| نوع الوثيقة: | article |
| وصف الملف: | electronic resource |
| اللغة: | English |
| تدمد: | 2050-084X |
| Relation: | https://elifesciences.org/articles/60038; https://doaj.org/toc/2050-084X |
| DOI: | 10.7554/eLife.60038 |
| URL الوصول: | https://doaj.org/article/25320fe37bca42ca940a58711529e988 |
| رقم الانضمام: | edsdoj.25320fe37bca42ca940a58711529e988 |
| قاعدة البيانات: | Directory of Open Access Journals |
Full Text Finder | تدمد: | 2050084X |
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| DOI: | 10.7554/eLife.60038 |