Academic Journal
Role of ADP ribosylation factor guanylate kinase 1 in the malignant biological behavior of gastric cancer
| العنوان: | Role of ADP ribosylation factor guanylate kinase 1 in the malignant biological behavior of gastric cancer |
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| المؤلفون: | Qiong Luo, Suyun Zhang, Fan Yang, Rui Feng, Qian Xu, Xiangqi Chen, Sheng Yang |
| المصدر: | Heliyon, Vol 10, Iss 12, Pp e33255- (2024) |
| بيانات النشر: | Elsevier, 2024. |
| سنة النشر: | 2024 |
| المجموعة: | LCC:Science (General) LCC:Social sciences (General) |
| مصطلحات موضوعية: | ASAP1, Gastric cancer, Overexpression, Knockdown, Mechanism, Biological behavior, Science (General), Q1-390, Social sciences (General), H1-99 |
| الوصف: | Aim: This study aims to investigate the influence of ASAP1 (ADP ribosylation factor guanylate kinase 1) on the malignant behavior of gastric cancer (GC) cells and to elucidate the potential molecular mechanisms involved in cancer development and progression. Methods: We assessed the impact of ASAP1 overexpression and knockdown on GC cell malignancy using CCK8, colony formation, flow cytometry (Annexin V/propidium iodide), Transwell migration, invasion, and scratch assays. Western blot analysis was used to assess the effects of ASAP1 on angiogenesis, matrix metalloproteinases (MMPs), apoptotic proteins, epithelial-mesenchymal transition (EMT)-related proteins, as well as AKT and p-AKT. The influence of ASAP1 knockdown was also evaluated in nude mice bearing BGC823 cell-derived tumors. Results: Our findings revealed that ASAP1 was significantly overexpressed in GC cells, enhancing their proliferation, invasion, and migration, while reducing apoptosis. Conversely, ASAP1 knockdown reversed these effects, markedly increasing the expression of cleaved-caspase 3 (Casp3), PARP, and the epithelial marker E-cadherin, and significantly decreasing MMP2, MMP9, VEGFA, and mesenchymal markers such as N-cadherin and vimentin. Additionally, it reduced AKT, and p-AKT levels (P |
| نوع الوثيقة: | article |
| وصف الملف: | electronic resource |
| اللغة: | English |
| تدمد: | 2405-8440 |
| Relation: | http://www.sciencedirect.com/science/article/pii/S2405844024092867; https://doaj.org/toc/2405-8440 |
| DOI: | 10.1016/j.heliyon.2024.e33255 |
| URL الوصول: | https://doaj.org/article/24b270b7daf848f0aec7466b8011de5f |
| رقم الانضمام: | edsdoj.24b270b7daf848f0aec7466b8011de5f |
| قاعدة البيانات: | Directory of Open Access Journals |
Full Text Finder | تدمد: | 24058440 |
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| DOI: | 10.1016/j.heliyon.2024.e33255 |