التفاصيل البيبلوغرافية
| العنوان: |
Characterization of Escherichia coli strains isolated from geese by detection of integron-mediated antimicrobial resistance |
| المؤلفون: |
Wanying Sun, Dongyang Wang, Shuang Yan, Yuan Xue |
| المصدر: |
Journal of Global Antimicrobial Resistance, Vol 31, Iss , Pp 10-14 (2022) |
| بيانات النشر: |
Elsevier, 2022. |
| سنة النشر: |
2022 |
| المجموعة: |
LCC:Microbiology |
| مصطلحات موضوعية: |
Goose, E. coli, Drug resistance, Integration, Gene cassettes, Microbiology, QR1-502 |
| الوصف: |
ABSTRACT: Objectives: Current research shows that the resistance of Escherichia coli (E. coli) is mainly related to integron gene cassettes. To assess the resistance of E. coli of goose origin and the carriage of its integron genes in four farms in Heilongjiang Province, antibiotic resistance phenotypes and the presence of various types of integrons were investigated. Methods: In this study, test strains were sampled and isolated from the farms, and 109 test strains were tested for drug sensitivity of 15 different antimicrobial drugs by the Kb disc diffusion method. Polymerase chain reaction was used to detect E. coli in three types of integrase genes (intI1, intI2, and intI3) and for sequencing analysis of the class I integron gene cassette. Results: Susceptibility test results show that more than 70% of tested strains exhibit resistant phenotypes to ampicillin, amoxicillin, imipenem, tetracycline, and doxycycline. The detection rate of class I integrons was 68.91%, while class II integrons and class III integrons were not detected. The detection rate of class I integrin gene cassette was 7.42%. Sequence analysis showed that strains carried different integron gene cassettes: dfrA17-aadA5, dfrA1-aadA1, dfrA27-arr-3, and aminoglycoside 3′'-nucleotidyltransferase. Conclusions: Results suggest that the detection rate of class I integrons is highly correlated with their drug resistance. Class I integrons provide a valuable guide to studying the spread and the expression of resistance genes and thus finding effective measures to prevent bacterial resistance. |
| نوع الوثيقة: |
article |
| وصف الملف: |
electronic resource |
| اللغة: |
English |
| تدمد: |
2213-7165 |
| Relation: |
http://www.sciencedirect.com/science/article/pii/S221371652200203X; https://doaj.org/toc/2213-7165 |
| DOI: |
10.1016/j.jgar.2022.08.013 |
| URL الوصول: |
https://doaj.org/article/24882e4600dc49dda056d37f9573c74e |
| رقم الانضمام: |
edsdoj.24882e4600dc49dda056d37f9573c74e |
| قاعدة البيانات: |
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