التفاصيل البيبلوغرافية
| العنوان: |
A novel function and mechanism of ischemia-induced retinal astrocyte-derived exosomes for RGC apoptosis of ischemic retinopathy |
| المؤلفون: |
Xiaoyuan Ye, Yunfei Liu, Congying Chen, Yimeng Sun, Fan Li, Yunzhao Fu, Jiawen Luo, Lishi Su, Wei Chi |
| المصدر: |
Molecular Therapy: Nucleic Acids, Vol 35, Iss 2, Pp 102209- (2024) |
| بيانات النشر: |
Elsevier, 2024. |
| سنة النشر: |
2024 |
| المجموعة: |
LCC:Therapeutics. Pharmacology |
| مصطلحات موضوعية: |
MT: Non-coding RNAs, retinal ischemia, astrocytes, exosomes, microRNA, MAPK8, Therapeutics. Pharmacology, RM1-950 |
| الوصف: |
Retinal ischemia is a common clinical event leading to retinal ganglion cell (RGC) death, resulting in irreversible vision loss. In the retina, glia-neuron communication is crucial for multiple functions and homeostasis. Extracellular vesicles, notably exosomes, play a critical role. The functions and mechanisms of retinal astrocyte-secreted exosomes remain unclear. Here, we isolated astrocyte-derived exosomes under hypoxia or normoxia and explored their role in an in vivo retinal ischemia-reperfusion (RIR) model. We found that hypoxia triggered astrocytes to produce a significantly increased number of exosomes, which could be internalized by RGCs in vivo or in vitro. Also, in the RIR model, the hypoxia-induced exosomes ameliorated the RIR injury and suppressed the RGC apoptosis. Furthermore, microRNA sequencing of retinal astrocyte-secreted exosomes revealed different patterns of exosomal miRNAs under hypoxia, particularly enriched with miR-329-5p. We verified that miR-329-5p was specifically bound to mitogen-activated protein kinase 8 mRNA, and subsequent JNK-pathway molecules were downregulated. We anticipated that the miR-329-5p/JNK pathway is a key to suppressing RGC apoptosis and preventing RIR injury. Such findings provided insights into the therapeutic potential of hypoxia-induced astrocyte-secreted exosomes and the miR-329-5p for treating retina ischemic diseases. |
| نوع الوثيقة: |
article |
| وصف الملف: |
electronic resource |
| اللغة: |
English |
| تدمد: |
2162-2531 |
| Relation: |
http://www.sciencedirect.com/science/article/pii/S2162253124000969; https://doaj.org/toc/2162-2531 |
| DOI: |
10.1016/j.omtn.2024.102209 |
| URL الوصول: |
https://doaj.org/article/246840d0a5f940fe893dbef0302bb72c |
| رقم الانضمام: |
edsdoj.246840d0a5f940fe893dbef0302bb72c |
| قاعدة البيانات: |
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