Academic Journal
TRPM8 channel inhibitor-encapsulated hydrogel as a tunable surface for bone tissue engineering
| العنوان: | TRPM8 channel inhibitor-encapsulated hydrogel as a tunable surface for bone tissue engineering |
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| المؤلفون: | Tusar Kanta Acharya, Satish Kumar, Nikhil Tiwari, Arijit Ghosh, Ankit Tiwari, Subhashis Pal, Rakesh Kumar Majhi, Ashutosh Kumar, Rashmita Das, Abhishek Singh, Pradip K. Maji, Naibedya Chattopadhyay, Luna Goswami, Chandan Goswami |
| المصدر: | Scientific Reports, Vol 11, Iss 1, Pp 1-16 (2021) |
| بيانات النشر: | Nature Portfolio, 2021. |
| سنة النشر: | 2021 |
| المجموعة: | LCC:Medicine LCC:Science |
| مصطلحات موضوعية: | Medicine, Science |
| الوصف: | Abstract A major limitation in the bio-medical sector is the availability of materials suitable for bone tissue engineering using stem cells and methodology converting the stochastic biological events towards definitive as well as efficient bio-mineralization. We show that osteoblasts and Bone Marrow-derived Mesenchymal Stem Cell Pools (BM-MSCP) express TRPM8, a Ca2+-ion channel critical for bone-mineralization. TRPM8 inhibition triggers up-regulation of key osteogenesis factors; and increases mineralization by osteoblasts. We utilized CMT:HEMA, a carbohydrate polymer-based hydrogel that has nanofiber-like structure suitable for optimum delivery of TRPM8-specific activators or inhibitors. This hydrogel is ideal for proper adhesion, growth, and differentiation of osteoblast cell lines, primary osteoblasts, and BM-MSCP. CMT:HEMA coated with AMTB (TRPM8 inhibitor) induces differentiation of BM-MSCP into osteoblasts and subsequent mineralization in a dose-dependent manner. Prolonged and optimum inhibition of TRPM8 by AMTB released from the gels results in upregulation of osteogenic markers. We propose that AMTB-coated CMT:HEMA can be used as a tunable surface for bone tissue engineering. These findings may have broad implications in different bio-medical sectors. |
| نوع الوثيقة: | article |
| وصف الملف: | electronic resource |
| اللغة: | English |
| تدمد: | 2045-2322 |
| Relation: | https://doaj.org/toc/2045-2322 |
| DOI: | 10.1038/s41598-021-81041-w |
| URL الوصول: | https://doaj.org/article/244af8698191426da6e6ef3ea0c07e0a |
| رقم الانضمام: | edsdoj.244af8698191426da6e6ef3ea0c07e0a |
| قاعدة البيانات: | Directory of Open Access Journals |
Full Text Finder | تدمد: | 20452322 |
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| DOI: | 10.1038/s41598-021-81041-w |