Academic Journal
Encapsulation of methylene blue in nucleoside-lipid-based liposomes: physicochemical characterization and evaluation for photodynamic therapy
| العنوان: | Encapsulation of methylene blue in nucleoside-lipid-based liposomes: physicochemical characterization and evaluation for photodynamic therapy |
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| المؤلفون: | Koffi Kowouvi, Alexis Verger, Alexandra Gaubert, Philippe Barthelemy, Gilles Dollo, Nolwenn Brandhonneur |
| المصدر: | Journal Africain de Technologie Pharmaceutique et Biopharmacie, Vol 3, Iss 2 (2024) |
| بيانات النشر: | Société Ouest Africaine de Pharmacie Galénique et Industrielle, 2024. |
| سنة النشر: | 2024 |
| المجموعة: | LCC:Pharmaceutical industry |
| مصطلحات موضوعية: | bleu de methylene, nanotechnologie, thérapie photodynamique, cancer ovarien, Pharmaceutical industry, HD9665-9675 |
| الوصف: | Methylene blue liposomes (MB-LPs) intended for photodynamic therapy (PDT) of ovarian cancer were formulated with a negatively charged nucleolipid and phosphatidylcholine using the lipidic film technique followed by an extrusion step. MB-LPs were characterized using dynamic light scattering (DLS), zeta potential (ZP), transmission electron microscopy (TEM), colloidal stability, Fourier-transform infra-red spectroscopy (FT-IR), UV-Visible spectroscopy and cellular studies. MB-LPs were spherical nano-objects with sizes obtained by DLS and TEM between 120 and 130 nm. MB-LPs showed a PDI near 0.10, a negative zeta potential near -42 mV and an encapsulation efficiency around 57 %. Characteristic peaks from ionic interactions of nucleolipids encapsulation of methylene blue were confirmed by FT-IR. Colloidal stability of MB-LPs was slightly affected in complex ionic media, sizes, PDI and ZP remained stable for 4 weeks at all storage temperature tested, allowing a potential use for in vitro and in vivo assays. The use of MB-LPs allowed slightly higher PDT activity based on the production of ROS against SKOV-3 ovarian cancer as compared to MB alone, a reduction of MB dark toxicity and a better protection against oxidation-reduction reactions. |
| نوع الوثيقة: | article |
| وصف الملف: | electronic resource |
| اللغة: | English French |
| تدمد: | 2756-7362 |
| Relation: | https://soapgi-jatpb.org/index.php/jatpb/article/view/185; https://doaj.org/toc/2756-7362 |
| DOI: | 10.57220/jatpb.v3i2.185 |
| URL الوصول: | https://doaj.org/article/23da3c45cd0a4d278143bb5b67f54ca1 |
| رقم الانضمام: | edsdoj.23da3c45cd0a4d278143bb5b67f54ca1 |
| قاعدة البيانات: | Directory of Open Access Journals |
| تدمد: | 27567362 |
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| DOI: | 10.57220/jatpb.v3i2.185 |