التفاصيل البيبلوغرافية
| العنوان: |
Prediction of Mycobacterium tuberculosis drug resistance by nucleotide MALDI-TOF-MS |
| المؤلفون: |
Xiaocui Wu, Guangkun Tan, Jinghui Yang, Yinjuan Guo, Chengchen Huang, Wei Sha, Fangyou Yu |
| المصدر: |
International Journal of Infectious Diseases, Vol 121, Iss , Pp 47-54 (2022) |
| بيانات النشر: |
Elsevier, 2022. |
| سنة النشر: |
2022 |
| المجموعة: |
LCC:Infectious and parasitic diseases |
| مصطلحات موضوعية: |
Drug-resistant tuberculosis, Nucleotide MALDI-TOF-MS, Antimicrobial susceptibility testing, Drug resistance, Infectious and parasitic diseases, RC109-216 |
| الوصف: |
ABSTRACT: Objectives: To evaluate the performance of nucleotide matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF-MS) in predicting the drug resistance of Mycobacterium tuberculosis. Methods: A total of 115 rifampin-resistant and 53 rifampin-susceptible tuberculosis (TB) clinical isolates were randomly selected from TB strains stored at -80℃ in the clinical laboratory of Shanghai Pulmonary Hospital. Nucleotide MALDI-TOF-MS was performed to predict the drug resistance using phenotypic susceptibility as the gold standard. Results: The overall assay sensitivities and specificities of nucleotide MALDI-TOF-MS were 92.2% and 100.0% for rifampin, 90.9% and 98.6% for isoniazid, 71.4% and 81.2% for ethambutol, 85.1% and 93.1% for streptomycin, 94.0% and 100.0% for amikacin, 77.8% and 99.3% for kanamycin, 75.0% and 93.3% for ofloxacin, and 75.0% and 93.3% for moxifloxacin. The concordances between nucleotide MALDI-TOF-MS antimicrobial susceptibility testing (AST) and phenotypic AST were 94.6% (rifampin), 90.1% (isoniazid), 79.2% (ethambutol), 89.9% (streptomycin), 99.4% (amikacin), 97.0% (kanamycin), 88.1% (ofloxacin), and 88.0% (moxifloxacin). Conclusion: Nucleotide MALDI-TOF-MS could be a promising tool for rapid detection of Mycobacterium tuberculosis drug sensitivity to rifampin, isoniazid, ethambutol, streptomycin, amikacin, kanamycin, ofloxacin, and moxifloxacin. |
| نوع الوثيقة: |
article |
| وصف الملف: |
electronic resource |
| اللغة: |
English |
| تدمد: |
1201-9712 |
| Relation: |
http://www.sciencedirect.com/science/article/pii/S1201971222002612; https://doaj.org/toc/1201-9712 |
| DOI: |
10.1016/j.ijid.2022.04.061 |
| URL الوصول: |
https://doaj.org/article/23ce5adc2ea442928c0753023f54029c |
| رقم الانضمام: |
edsdoj.23ce5adc2ea442928c0753023f54029c |
| قاعدة البيانات: |
Directory of Open Access Journals |
Full Text Finder