التفاصيل البيبلوغرافية
| العنوان: |
Evaluation of the Luciferase Assay-Based In Vitro Elicitation Test for Serum IgE |
| المؤلفون: |
Ryosuke Nakamura, Ayano Ishiwatari, Masakazu Higuchi, Yoshimi Uchida, Rika Nakamura, Hiroshi Kawakami, Atsuo Urisu, Reiko Teshima |
| المصدر: |
Allergology International, Vol 61, Iss 3, Pp 431-437 (2012) |
| بيانات النشر: |
Elsevier, 2012. |
| سنة النشر: |
2012 |
| المجموعة: |
LCC:Immunologic diseases. Allergy |
| مصطلحات موضوعية: |
allergen-specific IgE, allergy diagnosis, egg white allergy, luciferase, mast cells, Immunologic diseases. Allergy, RC581-607 |
| الوصف: |
Background: An in vitro elicitation test employing human high-affinity IgE receptor-expressing rat mast cell lines appears to be a useful method for measuring mast cell activation using a patient's IgE and an allergen; however, such cell lines are sensitive to human complements in the serum. We have recently developed a new luciferase-reporting mast cell line (RS-ATL8) to detect IgE crosslinking-induced luciferase expression (EXiLE) with relatively low quantities of serum IgE. Methods: A total of 30 patients suspected of having egg white (EW) allergy were subjected to an oral food challenge (OFC) test; then, the performances of EW-specific serum IgE (CAP-FEIA), EW-induced degranulation, and EXiLE responses in RS-ATL8 cells were compared using receiver-operating characteristic (ROC) curve analysis. The patients' sera were diluted to 1:100, which causes no cytotoxicity when sensitizing the RS-ATL8 cells for the degranulation and EXiLE tests. Results: The area under the ROC curves was highest in the EXiLE test (0.977), followed by CAP-FEIA (0.926) and degranulation (0.810). At an optimal cutoff range (1.648-1.876) calculated from the ROC curve of the EXiLE test, sensitivity and specificity were 0.944 and 0.917, respectively. A 95% positive predictive value was given at a cutoff level of 2.054 (fold increase in luciferase expression) by logistic regression analysis. Conclusions: In contrast to in vivo tests, the EXiLE test appears to be a useful tool in diagnosing patients suspected of having IgE-dependent EW allergy without the risk of severe systemic reactions. |
| نوع الوثيقة: |
article |
| وصف الملف: |
electronic resource |
| اللغة: |
English |
| تدمد: |
1323-8930 |
| Relation: |
http://www.sciencedirect.com/science/article/pii/S1323893015302392; https://doaj.org/toc/1323-8930 |
| DOI: |
10.2332/allergolint.11-OA-0407 |
| URL الوصول: |
https://doaj.org/article/239dc568270544f2948ea74f7d344d6d |
| رقم الانضمام: |
edsdoj.239dc568270544f2948ea74f7d344d6d |
| قاعدة البيانات: |
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