Academic Journal
Crosslinked duplex DNA nanogels that target specified proteins
| العنوان: | Crosslinked duplex DNA nanogels that target specified proteins |
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| المؤلفون: | Yasuhiko Iwasaki, Jun-ichi Kondo, Akinori Kuzuya, Rui Moriyama |
| المصدر: | Science and Technology of Advanced Materials, Vol 17, Iss 1, Pp 285-292 (2016) |
| بيانات النشر: | Taylor & Francis Group, 2016. |
| سنة النشر: | 2016 |
| المجموعة: | LCC:Materials of engineering and construction. Mechanics of materials LCC:Biotechnology |
| مصطلحات موضوعية: | biomarker, nanogel, phospholipid polymer, oligonucleotide aptamer, Materials of engineering and construction. Mechanics of materials, TA401-492, Biotechnology, TP248.13-248.65 |
| الوصف: | Specific detection of protein biomarkers plays an important role in diagnostics and therapeutics. We have fabricated polymeric nanogels, which can specifically interact with the cancer biomarker thrombin to serve as a model. Two types of 2-methacryloyloxyethyl phosphorylcholine (MPC) copolymers bearing a thrombin-binding oligonucleotide aptamer and its complementary chain were independently synthesized by redox-initiated radical polymerization. These MPC polymers associate in a complimentary fashion due to double strand formation of the oligonucleotides in aqueous media, leading to the spontaneous formation of spherical nanogels. Nanogel formation was confirmed by dynamic light scattering (DLS) and transmittance microscopy. The average size of nanogel particles was 124 ± 2 nm and the nanogels were mono-dispersed (polydispersity index 0.21). Functional intercalators could be stably incorporated into nanogels through the physical interaction between the intercalators and the oligonucleotides. The ethidium bromide (EtBr)-incorporating nanogels were used as detectors for thrombin. The fluorescence intensity of solutions containing the EtBr-incorporating nanogels was decreased with an increase in the concentration of thrombin. The transformation of quadruplex–thrombin structure from complementary double-stranded structures resulted in the decrease in fluorescence intensity. In contrast, the intensity did not change when the nanogels were incubated with albumin. Thrombin is only one such model used to demonstrate this technique; oligonucleotide aptamers can be freely designed to interact with versatile bio-substances. Therefore, aptamer-crosslinked nanogels can be appropriate nanomaterials for disease diagnosis and therapy. |
| نوع الوثيقة: | article |
| وصف الملف: | electronic resource |
| اللغة: | English |
| تدمد: | 1468-6996 1878-5514 14686996 |
| Relation: | https://doaj.org/toc/1468-6996; https://doaj.org/toc/1878-5514 |
| DOI: | 10.1080/14686996.2016.1189798 |
| URL الوصول: | https://doaj.org/article/e234c7d3a0764352a1a8bdcde243efca |
| رقم الانضمام: | edsdoj.234c7d3a0764352a1a8bdcde243efca |
| قاعدة البيانات: | Directory of Open Access Journals |
Full Text Finder | تدمد: | 14686996 18785514 |
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| DOI: | 10.1080/14686996.2016.1189798 |