التفاصيل البيبلوغرافية
| العنوان: |
Staphylococcus aureus Lung Infection Results in Down-Regulation of Surfactant Protein-A Mainly Caused by Pro-Inflammatory Macrophages |
| المؤلفون: |
Elisabeth Schicke, Zoltán Cseresnyés, Knut Rennert, Vanessa Vau, Karoline Frieda Haupt, Franziska Hornung, Sandor Nietzsche, Fatina Swiczak, Michaela Schmidtke, Brigitte Glück, Mirijam Koch, Michael Schacke, Regine Heller, Alexander S. Mosig, Marc Thilo Figge, Christina Ehrhardt, Bettina Löffler, Stefanie Deinhardt-Emmer |
| المصدر: |
Microorganisms, Vol 8, Iss 4, p 577 (2020) |
| بيانات النشر: |
MDPI AG, 2020. |
| سنة النشر: |
2020 |
| المجموعة: |
LCC:Biology (General) |
| مصطلحات موضوعية: |
Staphylococcus aureus, pneumonia, surfactant protein-A, influenza A virus, human alveolus-on-a-chip, Biology (General), QH301-705.5 |
| الوصف: |
Pneumonia is the leading cause of hospitalization worldwide. Besides viruses, bacterial co-infections dramatically exacerbate infection. In general, surfactant protein-A (SP-A) represents a first line of immune defense. In this study, we analyzed whether influenza A virus (IAV) and/or Staphylococcus aureus (S. aureus) infections affect SP-A expression. To closely reflect the situation in the lung, we used a human alveolus-on-a-chip model and a murine pneumonia model. Our results show that S. aureus can reduce extracellular levels of SP-A, most likely attributed to bacterial proteases. Mono-epithelial cell culture experiments reveal that the expression of SP-A is not directly affected by IAV or S. aureus. Yet, the mRNA expression of SP-A is strongly down-regulated by TNF-α, which is highly produced by professional phagocytes in response to bacterial infection. By using the human alveolus-on-a-chip model, we show that the down-regulation of SP-A is strongly dependent on macrophages. In a murine model of pneumonia, we can confirm that S. aureus decreases SP-A levels in vivo. These findings indicate that (I) complex interactions of epithelial and immune cells induce down-regulation of SP-A expression and (II) bacterial mono- and super-infections reduce SP-A expression in the lung, which might contribute to a severe outcome of bacterial pneumonia. |
| نوع الوثيقة: |
article |
| وصف الملف: |
electronic resource |
| اللغة: |
English |
| تدمد: |
2076-2607 |
| Relation: |
https://www.mdpi.com/2076-2607/8/4/577; https://doaj.org/toc/2076-2607 |
| DOI: |
10.3390/microorganisms8040577 |
| URL الوصول: |
https://doaj.org/article/a226e55f1b55485da6d257f79a60e2da |
| رقم الانضمام: |
edsdoj.226e55f1b55485da6d257f79a60e2da |
| قاعدة البيانات: |
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