Academic Journal
UCHL1 promotes the proliferation of porcine granulosa cells by stabilizing CCNB1
| العنوان: | UCHL1 promotes the proliferation of porcine granulosa cells by stabilizing CCNB1 |
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| المؤلفون: | Shengjie Shi, Huan Yuan, Lutong Zhang, Lei Gao, Lili Zhao, Xiangfang Zeng, Shiyan Qiao, Guiyan Chu, Chuanjiang Cai |
| المصدر: | Journal of Animal Science and Biotechnology, Vol 15, Iss 1, Pp 1-14 (2024) |
| بيانات النشر: | BMC, 2024. |
| سنة النشر: | 2024 |
| المجموعة: | LCC:Animal culture LCC:Veterinary medicine |
| مصطلحات موضوعية: | CCNB1, Granulosa cells, Isovitexin, Proliferation, UCHL1, Animal culture, SF1-1100, Veterinary medicine, SF600-1100 |
| الوصف: | Abstract Background The proliferation of porcine ovarian granulosa cells (GCs) is essential to follicular development and the ubiquitin–proteasome system is necessary for maintaining cell cycle homeostasis. Previous studies found that the deubiquitinase ubiquitin carboxyl-terminal hydrolase 1 (UCHL1) regulates female reproduction, especially in ovarian development. However, the mechanism by which UCHL1 regulates porcine GC proliferation remains unclear. Results UCHL1 overexpression promoted GC proliferation, and knockdown had the opposite effect. UCHL1 is directly bound to cyclin B1 (CCNB1), prolonging the half-life of CCNB1 and inhibiting its degradation, thereby promoting GC proliferation. What’s more, a flavonoid compound-isovitexin improved the enzyme activity of UCHL1 and promoted the proliferation of porcine GCs. Conclusions UCHL1 promoted the proliferation of porcine GCs by stabilizing CCNB1, and isovitexin enhanced the enzyme activity of UCHL1. These findings reveal the role of UCHL1 and the potential of isovitexin in regulating proliferation and provide insights into identifying molecular markers and nutrients that affect follicle development. |
| نوع الوثيقة: | article |
| وصف الملف: | electronic resource |
| اللغة: | English |
| تدمد: | 2049-1891 |
| Relation: | https://doaj.org/toc/2049-1891 |
| DOI: | 10.1186/s40104-024-01043-2 |
| URL الوصول: | https://doaj.org/article/2253e0a7c8d44a789bce741bea052f97 |
| رقم الانضمام: | edsdoj.2253e0a7c8d44a789bce741bea052f97 |
| قاعدة البيانات: | Directory of Open Access Journals |
Full Text Finder | تدمد: | 20491891 |
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| DOI: | 10.1186/s40104-024-01043-2 |