التفاصيل البيبلوغرافية
| العنوان: |
Identification of the critical residues responsible for differential reactivation of the triosephosphate isomerases of two trypanosomes |
| المؤلفون: |
Monica Rodríguez-Bolaños, Nallely Cabrera, Ruy Perez-Montfort |
| المصدر: |
Open Biology, Vol 6, Iss 10 (2016) |
| بيانات النشر: |
The Royal Society, 2016. |
| سنة النشر: |
2016 |
| المجموعة: |
LCC:Biology (General) |
| مصطلحات موضوعية: |
triosephosphate isomerase, critical residues, reactivation, protein folding, guanidine hydrochloride, site-directed mutagenesis, Biology (General), QH301-705.5 |
| الوصف: |
The reactivation of triosephosphate isomerase (TIM) from unfolded monomers induced by guanidine hydrochloride involves different amino acids of its sequence in different stages of protein refolding. We describe a systematic mutagenesis method to find critical residues for certain physico-chemical properties of a protein. The two similar TIMs of Trypanosoma brucei and Trypanosoma cruzi have different reactivation velocities and efficiencies. We used a small number of chimeric enzymes, additive mutants and planned site-directed mutants to produce an enzyme from T. brucei with 13 mutations in its sequence, which reactivates fast and efficiently like wild-type (WT) TIM from T. cruzi, and another enzyme from T. cruzi, with 13 slightly altered mutations, which reactivated slowly and inefficiently like the WT TIM of T. brucei. Our method is a shorter alternative to random mutagenesis, saturation mutagenesis or directed evolution to find multiple amino acids critical for certain properties of proteins. |
| نوع الوثيقة: |
article |
| وصف الملف: |
electronic resource |
| اللغة: |
English |
| تدمد: |
2046-2441 |
| Relation: |
https://doaj.org/toc/2046-2441 |
| DOI: |
10.1098/rsob.160161 |
| URL الوصول: |
https://doaj.org/article/2238e10ed84f4fdc87f9a9d657558586 |
| رقم الانضمام: |
edsdoj.2238e10ed84f4fdc87f9a9d657558586 |
| قاعدة البيانات: |
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