Academic Journal
Characterization of immunostimulatory pectic polysaccharide isolated from leaves of Diospyros kaki Thumb. (Persimmon)
| العنوان: | Characterization of immunostimulatory pectic polysaccharide isolated from leaves of Diospyros kaki Thumb. (Persimmon) |
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| المؤلفون: | Myoung-Sook Shin, Ho Lee, Hee-Do Hong, Kwang-Soon Shin |
| المصدر: | Journal of Functional Foods, Vol 26, Iss , Pp 319-329 (2016) |
| بيانات النشر: | Elsevier, 2016. |
| سنة النشر: | 2016 |
| المجموعة: | LCC:Nutrition. Foods and food supply |
| مصطلحات موضوعية: | Diospyros kaki Thumb. leaves, Polysaccharide, Macrophage activation, MAPK, NF-κB, Nutrition. Foods and food supply, TX341-641 |
| الوصف: | In this study, three polysaccharide fractions (PLE-I–III) were purified from crude polysaccharides from leaves of Diospyros kaki Thumb. (Persimmon) that were hydrolysed with pectinase, and their monosaccharide composition was analysed. Among the three fractions, PLE-I was found to markedly stimulate the immune activity (IL-6/IL-12 and TNF-α production) of peritoneal macrophages cells. Signal transduction studies showed that PLE-I phosphorylated MAPK and NF-κB in RAW264.7 cells in a concentration-dependent manner. The effect of PLE-I on IL-6 production was found to be completely suppressed by specific inhibitors of JNK (SP) and ERK (U0126), but not a p38 inhibitor. Moreover, PLE-I-induced phosphorylation of the transcription factor c-Jun was inhibited by SP and U0126 treatment, whereas iNOS expression was blocked by inhibitors of JNK, p38, and NF-κB. Collectively, these results suggest that PLE-I-induced macrophage activation is regulated via JNK/ERK and not p38, whereas iNOS expression is primarily regulated via MAPK and NF-κB. |
| نوع الوثيقة: | article |
| وصف الملف: | electronic resource |
| اللغة: | English |
| تدمد: | 1756-4646 74405829 |
| Relation: | http://www.sciencedirect.com/science/article/pii/S1756464616302316; https://doaj.org/toc/1756-4646 |
| DOI: | 10.1016/j.jff.2016.07.025 |
| URL الوصول: | https://doaj.org/article/214c4fb653924bf681a744058290f32c |
| رقم الانضمام: | edsdoj.214c4fb653924bf681a744058290f32c |
| قاعدة البيانات: | Directory of Open Access Journals |
| تدمد: | 17564646 74405829 |
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| DOI: | 10.1016/j.jff.2016.07.025 |