Academic Journal
A multimodal approach to diagnosis of neuromuscular neosporosis in dogs
| العنوان: | A multimodal approach to diagnosis of neuromuscular neosporosis in dogs |
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| المؤلفون: | Vanessa Alf, Federica Tirrito, Andrea Fischer, Rodolfo Cappello, Anna‐Mariam Kiviranta, Tanja A. Steinberg, Federica Poli, Felix Stotz, Omar V. Del Vecchio, Stefanie Dörfelt, Cristian Falzone, André Knittel, Shenja Loderstedt, Edy Mercuriali, Joana Tabanez, Paolo Zagarella, Kaspar Matiasek, Marco Rosati |
| المصدر: | Journal of Veterinary Internal Medicine, Vol 38, Iss 5, Pp 2561-2570 (2024) |
| بيانات النشر: | Wiley, 2024. |
| سنة النشر: | 2024 |
| المجموعة: | LCC:Veterinary medicine |
| مصطلحات موضوعية: | histopathology, immunohistochemistry, in situ hybridization, muscle biopsy, Neospora caninum, PCR, Veterinary medicine, SF600-1100 |
| الوصف: | Abstract Background Early diagnosis of neosporosis in dogs is challenging. Objectives To evaluate the feasibility of a compound multimodal testing approach for diagnosing in dogs neuromuscular and combined forms of neosporosis. Animals A total of 16 dogs diagnosed with solely neuromuscular neosporosis or with a combination of neuromuscular and central nervous system neosporosis. Methods Retrospective review of clinical signs, laboratory findings, treatment, and outcome with focus on the diagnostic utility of different tests. Development of a chromogenic in situ hybridization (ISH) assay for the identification of Neospora caninum in paraffin‐embedded muscle samples. Results 13/16 dogs had only neuromuscular signs of neosporosis, 3/16 had disease signs with concomitant central nervous system (CNS) involvement. Serology was performed in 15/16, with 10/15 showing titers >1 : 160 at admission. PCR on muscle samples detected N. caninum DNA in 11/16. Immunohistochemistry (IHC) detected N. caninum in 9/16 and ISH in 9/16. Histopathology revealed inflammatory myopathy in 10/16, necrotizing myopathy in 5/16, borderline changes in 1/16 and tachyzoites in 9/16. In 4 cases, N. caninum infection was confirmed with all 5 diagnostic methods, 3 cases with 4, 2 with 3, 6 with 2, and 1 animal with 1. Conclusions and Clinical Importance Diagnosis of N. caninum infection should rely on a multimodal diagnostic approach and negativity of 1 single test should not allow for exclusion. Serology in combination with direct parasite identification via histopathology, DNA via PCR, or both modalities, appears a reliable diagnostic approach. |
| نوع الوثيقة: | article |
| وصف الملف: | electronic resource |
| اللغة: | English |
| تدمد: | 1939-1676 0891-6640 |
| Relation: | https://doaj.org/toc/0891-6640; https://doaj.org/toc/1939-1676 |
| DOI: | 10.1111/jvim.17145 |
| URL الوصول: | https://doaj.org/article/1ff2ec1ea8524060ae2f1eabd5621eae |
| رقم الانضمام: | edsdoj.1ff2ec1ea8524060ae2f1eabd5621eae |
| قاعدة البيانات: | Directory of Open Access Journals |
Full Text Finder | تدمد: | 19391676 08916640 |
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| DOI: | 10.1111/jvim.17145 |