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Improving Chinese hamster ovary host cell protein ELISA using Ella®: an automated microfluidic platform

التفاصيل البيبلوغرافية
العنوان: Improving Chinese hamster ovary host cell protein ELISA using Ella®: an automated microfluidic platform
المؤلفون: Kathleen Van Manen-Brush, Jacob Zeitler, John R White, Paul Younge, Samantha Willis, Marisa Jones
المصدر: BioTechniques, Vol 69, Iss 3, Pp 186-192 (2020)
بيانات النشر: Taylor & Francis Group, 2020.
سنة النشر: 2020
المجموعة: LCC:Biology (General)
مصطلحات موضوعية: automated immunoassays, biopharmaceutical drug development, Chinese hamster ovary, ELISA, host cell proteins, immunoassays, Biology (General), QH301-705.5
الوصف: Chinese hamster ovary (CHO) cells are a mammalian cell line used in the production of therapeutic proteins. Host cell proteins (HCPs) are process-related impurities that are derived from the host cell expression system. During biopharmaceutical drug development, removal of HCPs is required. Enzyme-linked immunosorbent assay (ELISA) is a common technique to quantitate HCPs, but is a labor-intensive process that takes up to 7 h. Ella® is an automated instrument that utilizes microfluidics and glass nanoreactors to quantitate HCPs in 75 min using similar ELISA reagents. The antibodies and antigens are captured on three distinct glass nanoreactors, resulting in sensitive reproducible data. Our results indicate that Ella quantitates CHO HCPs with precision, accuracy, sensitivity and trends comparable with our traditional CHO HCP ELISA.
نوع الوثيقة: article
وصف الملف: electronic resource
اللغة: English
تدمد: 1940-9818
0736-6205
Relation: https://doaj.org/toc/0736-6205; https://doaj.org/toc/1940-9818
DOI: 10.2144/btn-2020-0074
URL الوصول: https://doaj.org/article/1fcc32b0ba1645b7b6328b8fe1c3cbf3
رقم الانضمام: edsdoj.1fcc32b0ba1645b7b6328b8fe1c3cbf3
قاعدة البيانات: Directory of Open Access Journals
الوصف
تدمد:19409818
07366205
DOI:10.2144/btn-2020-0074