Academic Journal
Accuracy of a new rapid diagnostic test for urinary antigen detection and assessment of drug treatment in opisthorchiasis
| العنوان: | Accuracy of a new rapid diagnostic test for urinary antigen detection and assessment of drug treatment in opisthorchiasis |
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| المؤلفون: | Chanika Worasith, Jiraporn Sithithaworn, Phattharaphon Wongphutorn, Chutima Homwong, Kanoknan Khongsukwiwat, Anchalee Techasen, Kulthida Y. Kopolrat, Watcharin Loilome, Nisana Namwat, Bandit Thinkamrop, Chaiwat Tawarungruang, Attapol Titapun, Thewarach Laha, Ross H. Andrews, Simon D. Taylor-Robinson, Paiboon Sithithaworn |
| المصدر: | Infectious Diseases of Poverty, Vol 12, Iss 1, Pp 1-12 (2023) |
| بيانات النشر: | BMC, 2023. |
| سنة النشر: | 2023 |
| المجموعة: | LCC:Infectious and parasitic diseases LCC:Public aspects of medicine |
| مصطلحات موضوعية: | Liver fluke, Opisthorchis viverrini, Urinary antigen detection, Urinary Opisthorchis viverrini rapid diagnosis test, Enzyme-linked immunosorbent assay, Quantitative formalin-ethyl acetate concentration technique, Infectious and parasitic diseases, RC109-216, Public aspects of medicine, RA1-1270 |
| الوصف: | Abstract Background Screening for opisthorchiasis, a parasitic worm infection affecting many millions of people in Southeast Asia, has traditionally relied on faecal egg examination such as the formalin-ethyl acetate concentration technique (FECT) and Kato-Katz method. Although the urinary enzyme-linked immunosorbent assay (ELISA) has been used more recently, we developed a urinary antigen-based rapid diagnostic test (RDT) to simplify diagnosis and as a point-of-care testing (POCT) and field applications for surveillance and control of opisthorchiasis. Methods A urinary Opisthorchis viverrini (OV)-RDT was developed using immunochromatographic methodology with a specific monoclonal antibody against OV. The diagnostic performance of the urinary OV-RDT was compared to that of quantitative faecal FECT and urinary antigen ELISA (n = 493). Cross-reactivities of urinary OV-RDT with other helminthiases coexisted with O. viverrini were determined (n = 96). A field trial in the application of urinary OV-RDT was compared with urinary antigen ELISA at baseline screening and assessment of drug treatment outcomes in opisthorchiasis (n = 1629). The McNemar chi-square, Kruskal–Wallis and Cohen’s kappa coefficient (κ-value) tests were used for statistical analyses. Results Urinary OV-RDT had sensitivity of 94.2% and specificity of 93.2%, compared to faecal FECT. Urinary OV-RDT had high diagnostic agreement (Kappa = 0.842–0.874, P 0.8, P 0.05). Conclusions The urinary OV-RDT test has high potential as a new tool for screening and evaluating treatment outcomes in opisthorchiasis. The ease of sample collection and simplicity of urinary OV-RDT may facilitate mass screening, control and elimination of opisthorchiasis, thereby contributing to a reduction in the disease burden in Southeast Asia. Graphical Abstract |
| نوع الوثيقة: | article |
| وصف الملف: | electronic resource |
| اللغة: | English |
| تدمد: | 2049-9957 |
| Relation: | https://doaj.org/toc/2049-9957 |
| DOI: | 10.1186/s40249-023-01162-4 |
| URL الوصول: | https://doaj.org/article/e1f173d4b388476bb05fa9f1a9226789 |
| رقم الانضمام: | edsdoj.1f173d4b388476bb05fa9f1a9226789 |
| قاعدة البيانات: | Directory of Open Access Journals |
Full Text Finder | تدمد: | 20499957 |
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| DOI: | 10.1186/s40249-023-01162-4 |