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High-efficient serum-free differentiation of endothelial cells from human iPS cells

التفاصيل البيبلوغرافية
العنوان: High-efficient serum-free differentiation of endothelial cells from human iPS cells
المؤلفون: Sarkawt Hamad, Daniel Derichsweiler, John Antonydas Gaspar, Konrad Brockmeier, Jürgen Hescheler, Agapios Sachinidis, Kurt Paul Pfannkuche
المصدر: Stem Cell Research & Therapy, Vol 13, Iss 1, Pp 1-16 (2022)
بيانات النشر: BMC, 2022.
سنة النشر: 2022
المجموعة: LCC:Medicine (General)
LCC:Biochemistry
مصطلحات موضوعية: Human induced pluripotent stem cells, iPS cells, hiPSCs, Differentiation, Endothelial cells, Regenerative medicine, 2D monolayer culture, Medicine (General), R5-920, Biochemistry, QD415-436
الوصف: Abstract Introduction Endothelial cells (ECs) form the inner lining of all blood vessels of the body play important roles in vascular tone regulation, hormone secretion, anticoagulation, regulation of blood cell adhesion and immune cell extravasation. Limitless ECs sources are required to further in vitro investigations of ECs’ physiology and pathophysiology as well as for tissue engineering approaches. Ideally, the differentiation protocol avoids animal-derived components such as fetal serum and yields ECs at efficiencies that make further sorting obsolete for most applications. Method Human induced pluripotent stem cells (hiPSCs) are cultured under serum-free conditions and induced into mesodermal progenitor cells via stimulation of Wnt signaling for 24 h. Mesodermal progenitor cells are further differentiated into ECs by utilizing a combination of human vascular endothelial growth factor A165 (VEGF), basic fibroblast growth factor (bFGF), 8-Bromoadenosine 3′,5′-cyclic monophosphate sodium salt monohydrate (8Bro) and melatonin (Mel) for 48 h. Result This combination generates hiPSC derived ECs (hiPSC-ECs) at a fraction of 90.9 ± 1.5% and is easily transferable from the two-dimensional (2D) monolayer into three-dimensional (3D) scalable bioreactor suspension cultures. hiPSC-ECs are positive for CD31, VE-Cadherin, von Willebrand factor and CD34. Furthermore, the majority of hiPSC-ECs express the vascular endothelial marker CD184 (CXCR4). Conclusion The differentiation method presented here generates hiPSC-ECs in only 6 days, without addition of animal sera and at high efficiency, hence providing a scalable source of hiPSC-ECs.
نوع الوثيقة: article
وصف الملف: electronic resource
اللغة: English
تدمد: 1757-6512
Relation: https://doaj.org/toc/1757-6512
DOI: 10.1186/s13287-022-02924-x
URL الوصول: https://doaj.org/article/1e160f6c5ed2454b8d053c9df50c2f23
رقم الانضمام: edsdoj.1e160f6c5ed2454b8d053c9df50c2f23
قاعدة البيانات: Directory of Open Access Journals
الوصف
تدمد:17576512
DOI:10.1186/s13287-022-02924-x