Academic Journal
The BMP signaling pathway enhances the osteoblastic differentiation of bone marrow mesenchymal stem cells in rats with osteoporosis
| العنوان: | The BMP signaling pathway enhances the osteoblastic differentiation of bone marrow mesenchymal stem cells in rats with osteoporosis |
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| المؤلفون: | Bin Zhao, Gengyan Xing, Aiyuan Wang |
| المصدر: | Journal of Orthopaedic Surgery and Research, Vol 14, Iss 1, Pp 1-8 (2019) |
| بيانات النشر: | BMC, 2019. |
| سنة النشر: | 2019 |
| المجموعة: | LCC:Orthopedic surgery LCC:Diseases of the musculoskeletal system |
| مصطلحات موضوعية: | BMP signaling pathway, BMP-2, Smad1, Osteoporosis, Bone marrow mesenchymal stem cells, Osteoblastic differentiation, Orthopedic surgery, RD701-811, Diseases of the musculoskeletal system, RC925-935 |
| الوصف: | Abstract Background This study was conducted with the aim of exploring the effect of the BMP signaling pathway on osteoblastic differentiation in rat bone marrow mesenchymal stem cells (rBMSCs) in rats with osteoporosis (OP). Methods The bilateral ovaries of female SD rats were resected for the establishment of a rat OP model. The osteoblastic differentiation of isolated rBMSCs was identified through osteogenic induction. Adipogenetic induction and flow cytometry (FCM) were used to detect adipogenic differentiation and the expression of rBMSC surface markers. The rBMSCs were grouped into the blank group, NC group, si-BMP2 group, and oe-BMP2 group. The expression levels of key factors and osteogenesis-related factors were determined by Western blot and quantitative real-time polymerase chain reaction (qRT-PCR). The formation of calcified nodules was observed by alizarin red staining. ALP activity was measured by alkaline phosphatase staining. Results The rats with OP had greater weight but decreased bone mineral density (BMD) than normal rats (all P |
| نوع الوثيقة: | article |
| وصف الملف: | electronic resource |
| اللغة: | English |
| تدمد: | 1749-799X |
| Relation: | https://doaj.org/toc/1749-799X |
| DOI: | 10.1186/s13018-019-1512-3 |
| URL الوصول: | https://doaj.org/article/1dbb48f2176f4c9b83c6a57df9f15d21 |
| رقم الانضمام: | edsdoj.1dbb48f2176f4c9b83c6a57df9f15d21 |
| قاعدة البيانات: | Directory of Open Access Journals |
Full Text Finder | تدمد: | 1749799X |
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| DOI: | 10.1186/s13018-019-1512-3 |