Academic Journal
Wss1 metalloprotease partners with Cdc48/Doa1 in processing genotoxic SUMO conjugates
| العنوان: | Wss1 metalloprotease partners with Cdc48/Doa1 in processing genotoxic SUMO conjugates |
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| المؤلفون: | Maxim Y Balakirev, James E Mullally, Adrien Favier, Nicole Assard, Eric Sulpice, David F Lindsey, Anastasia V Rulina, Xavier Gidrol, Keith D Wilkinson |
| المصدر: | eLife, Vol 4 (2015) |
| بيانات النشر: | eLife Sciences Publications Ltd, 2015. |
| سنة النشر: | 2015 |
| المجموعة: | LCC:Medicine LCC:Science LCC:Biology (General) |
| مصطلحات موضوعية: | SUMO metabolism, DNA repair, poly SUMO, Cdc48, Doa1, vacuole, Medicine, Science, Biology (General), QH301-705.5 |
| الوصف: | Sumoylation during genotoxic stress regulates the composition of DNA repair complexes. The yeast metalloprotease Wss1 clears chromatin-bound sumoylated proteins. Wss1 and its mammalian analog, DVC1/Spartan, belong to minigluzincins family of proteases. Wss1 proteolytic activity is regulated by a cysteine switch mechanism activated by chemical stress and/or DNA binding. Wss1 is required for cell survival following UV irradiation, the smt3-331 mutation and Camptothecin-induced formation of covalent topoisomerase 1 complexes (Top1cc). Wss1 forms a SUMO-specific ternary complex with the AAA ATPase Cdc48 and an adaptor, Doa1. Upon DNA damage Wss1/Cdc48/Doa1 is recruited to sumoylated targets and catalyzes SUMO chain extension through a newly recognized SUMO ligase activity. Activation of Wss1 results in metalloprotease self-cleavage and proteolysis of associated proteins. In cells lacking Tdp1, clearance of topoisomerase covalent complexes becomes SUMO and Wss1-dependent. Upon genotoxic stress, Wss1 is vacuolar, suggesting a link between genotoxic stress and autophagy involving the Doa1 adapter. |
| نوع الوثيقة: | article |
| وصف الملف: | electronic resource |
| اللغة: | English |
| تدمد: | 2050-084X |
| Relation: | https://elifesciences.org/articles/06763; https://doaj.org/toc/2050-084X |
| DOI: | 10.7554/eLife.06763 |
| URL الوصول: | https://doaj.org/article/1d2327de45b944ef8e8e73046a85a414 |
| رقم الانضمام: | edsdoj.1d2327de45b944ef8e8e73046a85a414 |
| قاعدة البيانات: | Directory of Open Access Journals |
Full Text Finder | تدمد: | 2050084X |
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| DOI: | 10.7554/eLife.06763 |